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A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli

Ghigo, J M, Létoffé, S, Wandersman, C·1997·Journal of Bacteriology
DOI 10.1128/jb.179.11.3572-3579.1997
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Abstract

The utilization by Serratia marcescens of heme bound to hemoglobin requires HasA, an extracellular heme-binding protein. This unique heme acquisition system was studied in an Escherichia coli hemA mutant that was a heme auxotroph. We identified a 92-kDa iron-regulated S. marcescens outer membrane protein, HasR, which alone enabled the E. coli hemA mutant to grow on heme or hemoglobin as a porphyrin source. The concomitant secretion of HasA by the HasR-producing hemA mutant greatly facilitates the acquisition of heme from hemoglobin. This is the first report of a synergy between an outer membrane protein and an extracellular heme-binding protein, HasA, acting as a heme carrier, which we termed a hemophore.

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Metadata

Authors
Ghigo, J. M., Létoffé, S., & Wandersman, C.
Affiliations
Unité de Physiologie Cellulaire, Institut Pasteur (CNRS URA 1300), Paris, France.
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
1997-06
Volume
179
Issue
11
Pages
3572-3579
ISSN
  • 0021-9193
  • 1098-5530
DOI
10.1128/jb.179.11.3572-3579.1997
Cited by
164
References
1
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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CoChat. (2026). Citation check: A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli. https://app.cochat.ai/papers/10.1128/jb.179.11.3572-3579.1997
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Ghigo, J. M., Létoffé, S., & Wandersman, C. (1997). A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli. Journal of Bacteriology. https://doi.org/10.1128/jb.179.11.3572-3579.1997