The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae
Open accessAbstract
A 5.0-kbp EcoRV-EcoRI restriction fragment was cloned and analyzed from genomic DNA of Aeromonas caviae, a bacterium producing a copolyester of (R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyhexanoate (3HHx) [P(3HB-co-3HHx)] from alkanoic acids or oils. The nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (PHA) synthase gene (phaC(Ac) [i.e., the phaC gene from A. caviae]) together with four open reading frames (ORF1, -3, -4, and -5) and one putative promoter region. The cloned fragments could not only complement PHA-negative mutants of Alcaligenes eutrophus and Pseudomonas putida, but also confer the ability to synthesize P(3HB-co-3HHx) from octanoate or hexanoate on the mutants' hosts. Furthermore, coexpression of ORF1 and ORF3 genes with phaC(Ac) in the A. eutrophus mutant resulted in a decrease in the polyester content of the cells. Escherichia coli expressing ORF3 showed (R)-enoyl-coenzyme A (CoA) hydratase activity, suggesting that (R)-3-hydroxyacyl-CoA monomer units are supplied via the (R)-specific hydration of enoyl-CoA in A. caviae. The transconjugant of the A. eutrophus mutant expressing only phaC(Ac) effectively accumulated P(3HB-co-3HHx) up to 96 wt% of the cellular dry weight from octanoate in one-step cultivation.
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Cites
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Compilation and analysis of Escherichia coli promoter DNA sequences
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Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
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Microbial Synthesis and Characterization of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
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A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry
Chen, G.-Q. (2009) · Chemical Society Reviews · doi:10.1039/b812677c · Cited by 1,303
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Bioplastics from microorganisms
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Metadata
- Authors
- Fukui, T., & Doi, Y.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1997-08
- Volume
- 179
- Issue
- 15
- Pages
- 4821-4830
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1801659885
- Cited by
- 282
- References
- 38
- FWCI
- 12.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Fukui, T., & Doi, Y. (1997). Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. Journal of Bacteriology. https://doi.org/10.1128/jb.179.15.4821-4830.1997
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