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Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions
Open accessAbstract
Disruption of newly identified genes in the pathogen Candida albicans is a vital step in determination of gene function. Several gene disruption methods described previously employ long regions of homology flanking a selectable marker. Here, we describe disruption of C. albicans genes with PCR products that have 50 to 60 bp of homology to a genomic sequence on each end of a selectable marker. We used the method to disrupt two known genes, ARG5 and ADE2 , and two sequences newly identified through the Candida genome project, HRM101 and ENX3. HRM101 and ENX3 are homologous to genes in the conserved RIM101 (previously called RIM1 ) and PacC pathways of Saccharomyces cerevisiae and Aspergillus nidulans . We show that three independent hrm101/hrm101 mutants and two independent enx3/enx3 mutants are defective in filamentation on Spider medium. These observations argue that HRM101 and ENX3 sequences are indeed portions of genes and that the respective gene products have related functions.
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Citations
Cited by
Noble, S. M., & Johnson, A. D. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.2.298-309.2005 · Cited by 592
Candida albicans: A molecular revolution built on lessons from budding yeast
Berman, J., & Sudbery, P. E. (2002) · Nature Reviews Genetics · doi:10.1038/nrg948 · Cited by 578
Critical Role of Bcr1-Dependent Adhesins in C. albicans Biofilm Formation In Vitro and In Vivo
Nobile, C. J., Andes, D. R., Nett, J. E., Smith, F. J., Yue, F., Phan, Q.-T., Edwards, J. E., Filler, S. G., & Mitchell, A. P. (2006) · PLoS Pathogens · doi:10.1371/journal.ppat.0020063 · Cited by 507
Self-citationRegulation of Cell-Surface Genes and Biofilm Formation by the C. albicans Transcription Factor Bcr1p
Nobile, C. J., & Mitchell, A. P. (2005) · Current Biology · doi:10.1016/j.cub.2005.05.047 · Cited by 464
Self-citationStimulation of Chitin Synthesis Rescues Candida albicans from Echinocandins
Walker, L. A., Munro, C. A., de Bruijn, I., Lenardon, M. D., McKinnon, A., & Gow, N. A. R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000040 · Cited by 445
Metadata
- Authors
- Wilson, R. B., Davis, D., & Mitchell, A. P.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1999-03-15
- Volume
- 181
- Issue
- 6
- Pages
- 1868-1874
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2119507799
- Cited by
- 762
- References
- 0
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- green
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- Wilson, R. B., Davis, D., & Mitchell, A. P. (1999). Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions. Journal of Bacteriology. https://doi.org/10.1128/jb.181.6.1868-1874.1999
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
