Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms
Open accessAbstract
Unsaturated biofilms of Pseudomonas putida , i.e., biofilms grown in humid air, were analyzed by atomic force microscopy to determine surface morphology, roughness, and adhesion forces in the outer and basal cell layers of fresh and desiccated biofilms. Desiccated biofilms were equilibrated with a 75.5% relative humidity atmosphere, which is far below the relative humidity of 98 to 99% at which these biofilms were cultured. In sharp contrast to the effects of drying on biofilms grown in fluid, we observed that drying caused little change in morphology, roughness, or adhesion forces in these unsaturated biofilms. Surface roughness for moist and dry biofilms increased approximately linearly with increasing scan sizes. This indicated that the divides between bacteria contributed more to overall roughness than did extracellular polymeric substances (EPS) on individual bacteria. The EPS formed higher-order structures we termed mesostructures. These mesostructures are much larger than the discrete polymers of glycolipids and proteins that have been previously characterized on the outer surface of these gram-negative bacteria.
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AFM imaging and force spectroscopy were carried out in an aqueous medium because dried biofilms change in morphology, roughness or adhesion forces compared to moist biofilms.
“AFM imaging and force spectroscopy were carried out in an aqueous medium because it has been shown that dried biofilms change in morphology, roughness or adhesion forces when compared to moist biofilms55.”
From Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms (2021) · cites this paper as [55]
The Discussion (page not provided) says the study's fresh and desiccated unsaturated biofilms had similar morphologies and that drying did not increase their surface roughness. The Results (page not provided) report no consistent difference in adhesion forces between dry and fresh biofilms. Although the Discussion notes that aquatic biofilms can shrink when dried, this paper's findings do not support the claimed changes in morphology, roughness, or adhesion forces as a reason to use an aqueous medium. (LLM, conf=0.90)
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Sorted by citations
Drame, I., Lafforgue, C., Formosa-Dague, C., Chapot-Chartier, M.-P., Piard, J.-C., Castelain, M., & Dague, E. (2021) · Scientific Reports · doi:10.1038/s41598-021-84030-1 · Cited by 28 · see citing works
- Authors
- Auerbach, I. D., Sorensen, C., Hansma, H. G., & Holden, P. A.
- Affiliations
- University of California, Santa Barbara (US)
- <!--label omitted: 1-->Donald Bren School of Environmental Science and Management1 and
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2000-07
- Volume
- 182
- Issue
- 13
- Pages
- 3809-3815
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC94554
- OpenAlex
- W2033798071
- Cited by
- 144
- References
- 56
- Citations by year
- 2026: 3 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 8 · 2019: 2 · 2018: 8 · 2017: 3 · 2016: 8 · 2015: 3 · 2014: 6 · 2013: 5 · 2012: 10
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- Pseudomonas putida, surface roughness, adhesion force, desiccation, relative humidity, extracellular polymeric substances, mesostructure, extracellular proteins, basal layer
- MeSH terms
- Pseudomonas putida, Biofilms, Microscopy, Atomic Force
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