The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function
Open accessAbstract
During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.
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“In patients with cystic fibrosis (CF), Pseudomonas aeruginosa (P. aeruginosa) establishes chronic airway infections that are dominated by biofilm growth.”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [4]
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“Mucoid conversion and increased alginate production alter the matrix and strengthen the chronic state of infection [3,4].”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [4]
In the citing paper
“Biofilms composed of cells overproducing alginate are highly structured and exhibit increased resistance to tobramycin78.”
From Pseudomonas aeruginosa: ecology, evolution, pathogenesis and antimicrobial susceptibility (2025) · cites this paper as [78]
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Citations
Cites
Bacterial Biofilms: A Common Cause of Persistent Infections
Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999) · Science · doi:10.1126/science.284.5418.1318 · Cited by 12,167
The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., & Greenberg, E. P. (1998) · Science · doi:10.1126/science.280.5361.295 · Cited by 3,396
Self-citationFlagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
O'Toole, G. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01062.x · Cited by 2,697
Molecular mechanisms of CFTR chloride channel dysfunction in cystic fibrosis
Welsh, M. J., & Smith, A. E. (1993) · Cell · doi:10.1016/0092-8674(93)90353-r · Cited by 1,485
Cited by
Pathophysiology and Management of Pulmonary Infections in Cystic Fibrosis
Gibson, R. L., Burns, J. L., & Ramsey, B. W. (2003) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200304-505so · Cited by 1,633
Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms
Karatan, E., & Watnick, P. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00041-08 · Cited by 1,048
A component of innate immunity prevents bacterial biofilm development
Singh, P. K., Parsek, M. R., Greenberg, E. P., & Welsh, M. J. (2002) · Nature · doi:10.1038/417552a · Cited by 1,026
Self-citationPseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation
Drenkard, E., & Ausubel, F. M. (2002) · Nature · doi:10.1038/416740a · Cited by 997
Ciofu, O., & Tolker-Nielsen, T. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00913 · Cited by 720
Metadata
- Authors
- Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M., & Parsek, M. R.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2001-09-15
- Volume
- 183
- Issue
- 18
- Pages
- 5395-5401
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2102677849
- Cited by
- 693
- References
- 4
- FWCI
- 8.03
- Type
- journal article
- Language
- en
- Open access
- green
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- Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M., & Parsek, M. R. (2001). Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function. Journal of Bacteriology. https://doi.org/10.1128/jb.183.18.5395-5401.2001
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
