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Homologues of Neisserial Heme Oxygenase in Gram-Negative Bacteria: Degradation of Heme by the Product of the pigA Gene of Pseudomonas aeruginosa

Ratliff, Melanie, Zhu, Wenming, Deshmukh, Rahul, Wilks, Angela, Stojiljkovic, Igor·2001·Journal of Bacteriology
DOI 10.1128/jb.183.21.6394-6403.2001
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Abstract

The oxidative cleavage of heme to release iron is a mechanism by which some bacterial pathogens can utilize heme as an iron source. The pigA gene of Pseudomonas aeruginosa is shown to encode a heme oxygenase protein, which was identified in the genome sequence by its significant homology (37%) with HemO of Neisseria meningitidis . When the gene encoding the neisserial heme oxygenase, hemO , was replaced with pigA , we demonstrated that pigA could functionally replace hemO and allow for heme utilization by neisseriae. Furthermore, when pigA was disrupted by cassette mutagenesis in P. aeruginosa , heme utilization was defective in iron-poor media supplemented with heme. This defect could be restored both by the addition of exogenous FeSO 4 , indicating that the mutant did not have a defect in iron metabolism, and by in trans complementation with pigA from a plasmid with an inducible promoter. The PigA protein was purified by ion-exchange chromotography. The UV-visible spectrum of PigA reconstituted with heme showed characteristics previously reported for other bacterial and mammalian heme oxygenases. The heme-PigA complex could be converted to ferric biliverdin in the presence of ascorbate, demonstrating the need for an exogenous reductant. Acidification and high-performance liquid chromatography analysis of the ascorbate reduction products identified a major product of biliverdin IX-β. This differs from the previously characterized heme oxygenases in which biliverdin IX-α is the typical product. We conclude that PigA is a heme oxygenase and may represent a class of these enzymes with novel regiospecificity.

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Metadata

Authors
Ratliff, M., Zhu, W., Deshmukh, R., Wilks, A., & Stojiljkovic, I.
Affiliations
  • <!--label omitted: 1-->Department of Microbiology and Immunology, Emory School of Medicine, Atlanta, Georgia 30322,1 and
  • <!--label omitted: 2-->Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, Maryland 212012
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
2001-11
Volume
183
Issue
21
Pages
6394-6403
ISSN
  • 0021-9193
  • 1098-5530
DOI
10.1128/jb.183.21.6394-6403.2001
Cited by
221
References
3
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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