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Complementation of the Essential Peptidoglycan Transpeptidase Function of Penicillin-Binding Protein 2 (PBP2) by the Drug Resistance Protein PBP2A in Staphylococcus aureus
Open accessAbstract
The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P spac promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 caused by inhibition of transcription of the pbp2 gene did not block growth of a methicillin-resistant S. aureus strain expressing the extra penicillin-binding protein PBP2A, a protein of extraspecies origin that is central to the mechanism of methicillin resistance. Several lines of evidence indicate that the essential function of PBP2 that can be compensated for by PBP2A is the transpeptidase activity. This provides direct genetic evidence that PBP2A has transpeptidase activity.
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Cited by
Bacterial Resistance to β-Lactam Antibiotics: Compelling Opportunism, Compelling Opportunity
Fisher, J. F., Meroueh, S. O., & Mobashery, S. (2005) · Chemical Reviews · doi:10.1021/cr030102i · Cited by 913
Oliveira, D. C., Tomasz, A., & de Lencastre, H. (2002) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(02)00227-x · Cited by 494
Self-citationVerdú-Expósito, C., Romanyk, J., Cuadros-González, J., TesfaMariam, A., Copa-Patiño, J. L., Pérez-Serrano, J., & Soliveri, J. (2020) · PLOS ONE · doi:10.1371/journal.pone.0230031 · Cited by 15
Metadata
- Authors
- Pinho, M. G., Filipe, S. R., de Lencastre, H., & Tomasz, A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2001-11-15
- Volume
- 183
- Issue
- 22
- Pages
- 6525-6531
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2139163053
- Cited by
- 225
- References
- 0
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Complementation of the Essential Peptidoglycan Transpeptidase Function of Penicillin-Binding Protein 2 (PBP2) by the Drug Resistance Protein PBP2A in Staphylococcus aureus. https://app.cochat.ai/papers/10.1128/jb.183.22.6525-6531.2001
- The paper
- Pinho, M. G., Filipe, S. R., de Lencastre, H., & Tomasz, A. (2001). Complementation of the Essential Peptidoglycan Transpeptidase Function of Penicillin-Binding Protein 2 (PBP2) by the Drug Resistance Protein PBP2A in Staphylococcus aureus. Journal of Bacteriology. https://doi.org/10.1128/jb.183.22.6525-6531.2001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
