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Cited by 15Antimicrobial Resistance in StaphylococcusStreptococcal Infections and TreatmentsAntibiotic Use and Resistance

Study of susceptibility to antibiotics and molecular characterization of high virulence Staphylococcus aureus strains isolated from a rural hospital in Ethiopia

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Verdú-Expósito, Cristina, Romanyk, Juan, Cuadros-González, Juan, TesfaMariam, Abraham, Copa-Patiño, José Luis, Pérez-Serrano, Jorge, Soliveri, Juan·2020·PLOS ONE
DOI 10.1371/journal.pone.0230031PMC PMC7067403
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Abstract

We characterised 80 Staphylococcus aureus strains isolated from human patients with SSTIs at a rural hospital in Ethiopia. Susceptibility to antibiotic of all strains was tested. The MLST method was used to type and a phylogenetic analysis was conducted employing the sequences of 7 housekeeping genes. PCR amplification was used to investigate the presence of the following virulence genes in all strains: hla (α-haemolysin), tstH (toxic shock syndrome toxin), luk PV (Panton-Valentine leukocidin), fnbA (fibronectin binding protein A) and mecA (methicillin resistance). Most of the strains were resistant to penicillin and ampicillin, but only 3 strains were resistant to oxacillin, and 1 of them was a true MRSA. The MLST results showed a high diversity of sequence types (ST), 55% of which were new, and ST152 was the most prevalent. A phylogeny study showed that many of the new STs were phylogenetically related to other previously described STs, but bore little relationship to the only ST from Ethiopia described in the database. Virulence gene detection showed a high prevalence of strains encoding the hla, fnbA and pvl genes (98.77%, 96.3% and 72.84%, respectively), a low prevalence of the tst gene (13.58%) and a markedly low prevalence of MRSA (1.25%). S. aureus strains isolated from patients in a rural area in Ethiopia showed low levels of antibiotic resistance, except to penicillin. Moreover, this study reveals new STs in Eastern Africa that are phylogenetically related to other previously described STs, and confirm the high prevalence of the pvl gene and the low prevalence of MRSA on the continent.

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  • The most prevalent MRSA genotypes in SSA have been demonstrated to be ST5, ST8, ST15, ST88, ST152, ST241, and ST239.

    “Moreover, the most prevalent genotypes have been demonstrated to be ST5, ST8, ST15, ST88, ST152, ST241, and ST239.162,[169][170][171]”

    From Antimicrobial Resistance Rates and Surveillance in Sub-Saharan Africa: Where Are We Now? (2022) · cites this paper as [162]

    The Results (Strain typing by MLST; page unavailable) identify ST152 as the most prevalent sequence type in this Ethiopian S. aureus sample and also report ST5 and ST15. The Discussion (page unavailable) cites an African review naming ST30, ST121 and ST152 as prevalent S. aureus types, explicitly describing those isolates as MSSA. Neither passage establishes the claimed seven-type list as the most prevalent MRSA genotypes across sub-Saharan Africa. (LLM, conf=0.82)

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Authors
Verdú-Expósito, C., Romanyk, J., Cuadros-González, J., TesfaMariam, A., Copa-Patiño, J. L., Pérez-Serrano, J., & Soliveri, J.
Affiliations
  • Universidad de Alcalá (ES)
  • Hospital Universitario Príncipe de Asturias (ES)
Corresponding author
Verdú-Expósito, Cristina
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2020-03-12
Volume
15
Issue
3
Pages
e0230031
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0230031
PMC
PMC7067403
OpenAlex
W3011108251
Cited by
15
References
1
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 1
FWCI
0.33
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Health Sciences › Medicine › Infectious Diseases › Streptococcal Infections and Treatments
  • Health Sciences › Medicine › Infectious Diseases › Antibiotic Use and Resistance
Keywords
Staphylococcus aureus, antibiotic susceptibility, methicillin resistance, virulence genes, Panton-Valentine leukocidin, alpha-hemolysin, toxic shock syndrome toxin, fibronectin-binding protein A, sequence types, molecular epidemiology, penicillin resistance, ampicillin resistance, oxacillin resistance, skin and soft tissue infections, rural hospitals, Ethiopia, new sequence types
MeSH terms
Ceftaroline, Anti-Bacterial Agents, Bacterial Proteins, Bacterial Toxins, Cephalosporins, Ethiopia, Exotoxins, Hospitals, Rural, Humans, Leukocidins, Microbial Sensitivity Tests, Penicillins, Phylogeny, Staphylococcal Infections, Staphylococcus aureus, Virulence, Drug Resistance, Bacterial, Methicillin-Resistant Staphylococcus aureus, Multilocus Sequence Typing

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