The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Bacillus anthracis IsdG, a Heme-Degrading Monooxygenase
Open accessAbstract
Bacillus anthracis, the causative agent of anthrax, utilizes hemin and hemoglobin for growth in culture, suggesting that these host molecules serve as sources for the nutrient iron during bacterial infection. Bioinformatic analyses of the B. anthracis genome revealed genes with similarity to the iron-regulated surface determinant (isd) system responsible for heme uptake in Staphylococcus aureus. We show that the protein product of one of these genes, isdG, binds hemin in a manner resembling the heme binding of known heme oxygenases. Formation of IsdG:hemin complexes in the presence of a suitable electron donor, e.g., ascorbate or cytochrome P450 reductase, promotes catalytic degradation of hemin to biliverdin with concomitant release of iron. IsdG is required for B. anthracis utilization of hemin as a sole iron source, and it is also necessary for bacterial protection against heme-mediated toxicity. These data suggest that IsdG functions as a heme-degrading monooxygenase in B. anthracis.
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“The Bacillus anthracis Isd proteins include IsdX1 and IsdX2, which are secreted haemophores that bind haemoglobin, haptoglobin-haemoglobin or haem 33-35 .”
From Nutritional immunity: the battle for nutrient metals at the host–pathogen interface (2022) · cites this paper as [35]
Experimental — accuracy not yet measuredIn the citing paper
“IsdDEF transports heme across the membrane for utilization by IsdG, an orthologue of the S. aureus heme oxygenase [133].”
From Heme Synthesis and Acquisition in Bacterial Pathogens (2016) · cites this paper as [133]
In the citing paper
“IsdG-family heme oxygenases have been characterized in S. lugdunensis, Bacillus anthracis, Bradyrhizobium japonicum, Brucella melitensis, and Mycobacterium tuberculosis [46, 50, 56–58].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [58]
In the citing paper
“While IsdG orthologs from each of these bacteria have been shown to bind and degrade heme, differences do exist.”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [58]
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Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
Dixon, T. C., Meselson, M. S., Guillemin, J. H., & Hanna, P. C. (1999) · New England Journal of Medicine · doi:10.1056/nejm199909093411107 · Cited by 871
The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria
Read, T. D., Peterson, S. N., Tourasse, N. J., Baillie, L. W. J., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen, J. A., Gill, S. R., et al. (2003) · Nature · doi:10.1038/nature01586 · Cited by 822
Opening the Iron Box: Transcriptional Metalloregulation by the Fur Protein
Escolar, L., Pérez-Martín, J., & de Lorenzo, V. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6223-6229.1999 · Cited by 815
The significance of iron in infection
Bullen, J. J. (1981) · Clinical Infectious Diseases · doi:10.1093/clinids/3.6.1127 · Cited by 603
Passage of Heme-Iron Across the Envelope of Staphylococcus aureus
Mazmanian, S. K., Skaar, E. P., Gaspar, A. H., Humayun, M., Gornicki, P., Jelenska, J., Joachmiak, A., Missiakas, D. M., & Schneewind, O. (2003) · Science · doi:10.1126/science.1081147 · Cited by 593
Self-citationCollier, R. J., & Young, J. A. T. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111301.140655 · Cited by 510
Demonstration of a capsule plasmid in Bacillus anthracis
Green, B. D., Battisti, L., Koehler, T. M., Thorne, C. B., & Ivins, B. E. (1985) · Infection and Immunity · doi:10.1128/iai.49.2.291-297.1985 · Cited by 491
Iron-Source Preference of Staphylococcus aureus Infections
Skaar, E. P., Humayun, M., Bae, T., DeBord, K. L., & Schneewind, O. (2004) · Science · doi:10.1126/science.1099930 · Cited by 440
Self-citationMazmanian, S. K., Ton-That, H., Su, K., & Schneewind, O. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032523999 · Cited by 372
Self-citation
Research works printed but not linked: 2
- 10.1016/S0022-2836(05)80360-2 · DOI · Paper page
- Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Press Cold Spring Harbor N.Y.
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Pi, H., Burroughs, O. S., Carlin, S. M., Beavers, W. N., Hillebrand, G. H., Krystofiak, E., Stauff, D. L., & Skaar, E. P. (2025) · mBio · doi:10.1128/mbio.02057-24 · Cited by 2
Self-citationSabet, R., Fassihi, A., & Moeinifard, B. (2009) · Research in Pharmaceutical Sciences · Cited by 2
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Grover, A., Nabi, A., Conger, M. A., & Liptak, M. D. (2026) · Dalton Transactions · doi:10.1039/d5dt02793d · Cited by 2
Pyne, A., Abduljalil, J. M. A., & Kwan, A. H. (2026) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2026.1842791 · Cited by 1
Carlson, P. E., Dixon, S. D., & Hanna, P. C. (2016) · ASM Press eBooks · doi:10.1128/9781555818524.ch16 · Cited by 1
Brunson, D. N., Manzer, H. S., Smith, A. B., Zackular, J. P., Kitten, T. O., & Lemos, J. A. C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.20.633879 · Cited by 1
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Parrow, N. L. (2010) · The Mathematics Enthusiast · Cited by 1
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Farrand, A. J., & Skaar, E. P. (2016) · ASM Press eBooks · doi:10.1128/9781555818524.ch5
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Rall, J., & Koehler, T. M. (2010) · Wiley eBooks · doi:10.1002/9780470891193.ch9
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Pishchany, G., & Skaar, E. P. (2010) · Wiley eBooks · doi:10.1002/9780470891193.ch7
Self-citationNovel insights into the action of antimicrobial agents against human pathogens
Tavares, A. F. N. (2013) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)
On the heme sensing system of Gram-positive bacterial pathogens
Stauff, D. L. (2009)
Inside or Out: Characterizing petrobactin use by Bacillus anthracis
Hagan, A. K. (2018) · Deep Blue (University of Michigan)
Hanna, P. C. (2019) · Molecular Microbiology · doi:10.1111/mmi.14363
The product and process of heme degradation in Staphylococcus aureus
Reniere, M. L. (2010)
Molecular Mechanism of Heme Acquisition and Degradation by the Human Pathogen Group A Streptococcus
Ouattara, M. (2022) · Digital Archive @ GSU · doi:10.57709/4056857
Variability of the iron uptake system components in Bacillus anthracis
Eremenko, E. I., Ryazanova, A. G., Pechkovskii, G. A., Aksenova, L. Y., & Semenova, O. V. (2026) · Journal of microbiology epidemiology immunobiology · doi:10.36233/0372-9311-726
Saillant, V. (2020) · theses.fr (ABES) · doi:10.70675/2c50a6ddz7c94z4048z81bdz04e5cce574fc
Ahmad, S., Rahman, H., Akram, M. S., Mehboob, J., Thomas, G. H., Alzahrani, K. J., Alsharif, K. F., & Alzahrani, F. M. (2026) · Microbial Pathogenesis · doi:10.1016/j.micpath.2026.108500 · Cited by 1
Metadata
- Authors
- Skaar, E. P., Gaspar, A. H., & Schneewind, O.
- Affiliations
- University of Chicago (US)
- Corresponding author
- Schneewind, Olaf
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2006-02
- Volume
- 188
- Issue
- 3
- Pages
- 1071-1080
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC1347327
- OpenAlex
- W2140572324
- Cited by
- 123
- References
- 61
- Citations by year
- 2026: 4 · 2025: 5 · 2023: 4 · 2022: 3 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 5 · 2016: 8 · 2015: 1 · 2014: 1 · 2013: 9 · 2012: 11
- FWCI
- 5.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Heart Association
- Great Lakes Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research (057153)
- U.S. Public Health Service
- National Institute of Allergy and Infectious Diseases (U54AI057153, AI38897, AI52474, R01AI038897, R01AI052474)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Bacillus anthracis, heme oxygenase, iron acquisition, heme binding, biliverdin, hemoglobin, anthrax
- MeSH terms
- Amino Acid Sequence, Bacillus anthracis, Heme, Heme Oxygenase (Decyclizing), Iron, Molecular Sequence Data, Oxygenases
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