DEPOLYMERIZATION OF POLY-β-HYDROXYBUTYRATE BY AN INTRACELLULAR ENZYME SYSTEM
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Merrick , J. M. (State University of New York, Buffalo), and M. Doudoroff . Depolymerization of poly-β-hydroxybutyrate by an intracellular enzyme system. J. Bacteriol. 88: 60–71. 1964.—The poly-β-hydroxybutyric acid contained in the “lipid granules” of Bacillus megaterium is hydrolyzed to d (−)-β-hydroxybutyric acid by a complex enzyme system present in the soluble enzyme fraction of polymer-depleted cells of Rhodospirillum rubrum . This system consists of a thermostable “activator,” a thermolabile “depolymerase,” and an “esterase.” Under certain conditions, the activator can be replaced by trypsin. Various chemical and physical treatments inactivate the “native lipid granules,” and make them unsuitable as a substrate for the digestive enzymes. This inactivation of the granules is often expressed in the extent rather than the rate of their digestion, and is not correlated with the destruction of the polymer-synthesizing enzymes associated with the granules. The principal product of depolymerase action is d (−)-β-hydroxybutyric acid, but small amounts of esterified products are also released. These are hydrolyzed by the esterase.
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PHB granules isolated from microbial cell extracts could be hydrolyzed to β-hydroxybutyric acid while the polymer was incubated with part of the cell extracts it had been purified from.
“Merrick, Doudoroff et al. noticed that polyhydroxybutyrate (PHB)”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [469]
Depolymerase and esterase could digest inclusion bodies of Bacillus megaterium containing PHB to produce D(-)-β-hydroxybutyric ester, with up to 90% hydrolysis after 100 min.
“depolymerase”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [469]
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- Authors
- Merrick, J. M., & Doudoroff, M.
- Affiliations
- University at Buffalo, State University of New York (US)
- University of California, Berkeley (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1964-07
- Volume
- 88
- Issue
- 1
- Pages
- 60-71
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC277257
- OpenAlex
- W2117373770
- Cited by
- 185
- References
- 6
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 4 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 4
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- polyhydroxybutyrate, depolymerization, Bacillus megaterium, Rhodospirillum rubrum, lipid granules, depolymerase, esterase, beta-hydroxybutyrate, enzyme activators, trypsin, polyhydroxyalkanoates
- MeSH terms
- Polyhydroxybutyrates, Bacillus megaterium, Cytoplasmic Granules, Enzymes, Esterases, Hydroxybutyrates, Metabolism, Polyesters, Polymers, Research, Rhodospirillum, Rhodospirillum rubrum, Trypsin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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