Cited by 185biodegradable polymer synthesis and propertiesMicrobial bioremediation and biosurfactantsLipid metabolism and biosynthesis

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DEPOLYMERIZATION OF POLY-β-HYDROXYBUTYRATE BY AN INTRACELLULAR ENZYME SYSTEM

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Merrick, J. M., Doudoroff, M.·1964·Journal of Bacteriology

DOI 10.1128/jb.88.1.60-71.1964PMC PMC277257

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Abstract

Merrick , J. M. (State University of New York, Buffalo), and M. Doudoroff . Depolymerization of poly-β-hydroxybutyrate by an intracellular enzyme system. J. Bacteriol. 88: 60–71. 1964.—The poly-β-hydroxybutyric acid contained in the “lipid granules” of Bacillus megaterium is hydrolyzed to d (−)-β-hydroxybutyric acid by a complex enzyme system present in the soluble enzyme fraction of polymer-depleted cells of Rhodospirillum rubrum . This system consists of a thermostable “activator,” a thermolabile “depolymerase,” and an “esterase.” Under certain conditions, the activator can be replaced by trypsin. Various chemical and physical treatments inactivate the “native lipid granules,” and make them unsuitable as a substrate for the digestive enzymes. This inactivation of the granules is often expressed in the extent rather than the rate of their digestion, and is not correlated with the destruction of the polymer-synthesizing enzymes associated with the granules. The principal product of depolymerase action is d (−)-β-hydroxybutyric acid, but small amounts of esterified products are also released. These are hydrolyzed by the esterase.

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Authors: Merrick, J. M., & Doudoroff, M.
Affiliations: University at Buffalo, State University of New York (US)
University of California, Berkeley (US)
Venue: Journal of Bacteriology
Publisher: American Society for Microbiology
Published: 1964-07
Volume: 88
Issue: 1
Pages: 60-71
ISSN: 0021-9193 (ISSN-L)
1098-5530
DOI: 10.1128/jb.88.1.60-71.1964
PMC: PMC277257
OpenAlex: W2117373770
Cited by: 185
References: 6
Citations by year: 2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 4 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 4
FWCI: 4.61
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Bacteriology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: polyhydroxybutyrate, depolymerization, Bacillus megaterium, Rhodospirillum rubrum, lipid granules, depolymerase, esterase, beta-hydroxybutyrate, enzyme activators, trypsin, polyhydroxyalkanoates
MeSH terms: Polyhydroxybutyrates, Bacillus megaterium, Cytoplasmic Granules, Enzymes, Esterases, Hydroxybutyrates, Metabolism, Polyesters, Polymers, Research, Rhodospirillum, Rhodospirillum rubrum, Trypsin

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DEPOLYMERIZATION OF POLY-β-HYDROXYBUTYRATE BY AN INTRACELLULAR ENZYME SYSTEM

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