CoChat
Sign Up
Cited by 185biodegradable polymer synthesis and propertiesMicrobial bioremediation and biosurfactantsLipid metabolism and biosynthesis

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

DEPOLYMERIZATION OF POLY-β-HYDROXYBUTYRATE BY AN INTRACELLULAR ENZYME SYSTEM

Open access
Merrick, J. M., Doudoroff, M.·1964·Journal of Bacteriology
DOI 10.1128/jb.88.1.60-71.1964PMC PMC277257
Open this paper in CoChat — free accountOpen PDF

Abstract

Merrick , J. M. (State University of New York, Buffalo), and M. Doudoroff . Depolymerization of poly-β-hydroxybutyrate by an intracellular enzyme system. J. Bacteriol. 88: 60–71. 1964.—The poly-β-hydroxybutyric acid contained in the “lipid granules” of Bacillus megaterium is hydrolyzed to d (−)-β-hydroxybutyric acid by a complex enzyme system present in the soluble enzyme fraction of polymer-depleted cells of Rhodospirillum rubrum . This system consists of a thermostable “activator,” a thermolabile “depolymerase,” and an “esterase.” Under certain conditions, the activator can be replaced by trypsin. Various chemical and physical treatments inactivate the “native lipid granules,” and make them unsuitable as a substrate for the digestive enzymes. This inactivation of the granules is often expressed in the extent rather than the rate of their digestion, and is not correlated with the destruction of the polymer-synthesizing enzymes associated with the granules. The principal product of depolymerase action is d (−)-β-hydroxybutyric acid, but small amounts of esterified products are also released. These are hydrolyzed by the esterase.

Overview

Integrity signals

Registry

Crossref · 10.1128/jb.88.1.60-71.1964

Cites

6 of 6 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 6 references

Research works printed but not linked: 2

  1. BOATMAN , E. S. , AND H. C. DOUGLAS. 1963 . Membrane of poly-,B-hydroxybutyrate granules of Rhodospirillum rubrum . J. Appl. Phys. 36 : 2528 .
  2. MERRICK , J. M. , F. P. DELAFIELD , A ND M . DOUDOROFF. 1962 . Hydrolysis of poly-,- hydroxybutyrate by intracellular and extracellular enzymes . Federation Proc. 21 : 228 .

Cited by

Sorted by citations

See all 185

Related

Metadata

Authors
Merrick, J. M., & Doudoroff, M.
Affiliations
  • University at Buffalo, State University of New York (US)
  • University of California, Berkeley (US)
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
1964-07
Volume
88
Issue
1
Pages
60-71
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
DOI
10.1128/jb.88.1.60-71.1964
PMC
PMC277257
OpenAlex
W2117373770
Cited by
185
References
6
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 4 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 4
FWCI
4.61
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Bacteriology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
polyhydroxybutyrate, depolymerization, Bacillus megaterium, Rhodospirillum rubrum, lipid granules, depolymerase, esterase, beta-hydroxybutyrate, enzyme activators, trypsin, polyhydroxyalkanoates
MeSH terms
Polyhydroxybutyrates, Bacillus megaterium, Cytoplasmic Granules, Enzymes, Esterases, Hydroxybutyrates, Metabolism, Polyesters, Polymers, Research, Rhodospirillum, Rhodospirillum rubrum, Trypsin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review