Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing
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In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.
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Previously a MATa to MATalpha switching event has been described for the P35_2/P35_3 pair.
“Previously a MATa to MATalpha switching event has been described already for the P35_2/P35_3 pair (Lin et al., 2007; Carreté et al., 2018).”
From Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations (2019) · cites this paper as Lin et al., 2007
C. glabrata shows genetic variability at the level of genomic nucleotide sequence.
“It has been reported that this fungus also shows genetic variability at the level of genomic nucleotide sequence (Lin et al., 2007).”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Lin et al., 2007
Candida glabrata has been frequently reported as exhibiting variable karyotypes between isolates.
“C. glabrata has been frequently reported as exhibiting variable karyotypes between isolates [57,58,59,60,61,62,63,64]”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [63]
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References 45 · Cited by 62
- Authors
- Lin, C.-Y., Chen, Y.-C., Lo, H.-J., Chen, K.-W., & Li, S.-Y.
- Affiliations
- Taiwan Centers for Disease Control (TW)
- National Taiwan University (TW)
- National Health Research Institutes (TW)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-08
- Volume
- 45
- Issue
- 8
- Pages
- 2452-2459
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC1951215
- OpenAlex
- W2006138081
- Cited by
- 62
- References
- 45
- Citations by year
- 2024: 2 · 2023: 3 · 2021: 3 · 2020: 1 · 2019: 7 · 2018: 2 · 2017: 5 · 2016: 2 · 2015: 2 · 2014: 7 · 2013: 3 · 2012: 6
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- National Institute on Deafness and Other Communication Disorders (R01DC002012, R01DC002014)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida glabrata, strain relatedness, multilocus sequence typing, pulsed-field gel electrophoresis, mating type, strain replacement, microvariation, HIV infection, intensive care unit isolates, fungal genotyping
- MeSH terms
- Candidiasis, Genotype, Humans, Cluster Analysis, Electrophoresis, Gel, Pulsed-Field, Mycological Typing Techniques, Sequence Analysis, DNA, Molecular Epidemiology, Candida glabrata, Genes, Mating Type, Fungal
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