Loop-Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
Open accessAbstract
Diagnosis of tuberculous meningitis (TBM) is often difficult. A reliable, simple, and rapid diagnostic test that can be performed in any standard laboratory could be helpful in TBM diagnosis. In this study, a loop-mediated isothermal amplification assay (LAMP) was evaluated to rapidly detect and diagnose TBM infection and was compared to the performance of nested PCR. Six specific primers were used to recognize the IS6110 genomic sequence from Mycobacterium tuberculosis, which included one forward outer primer, one reverse outer primer, two respective inner primers, and two loop primers. The optimum reaction temperature and time were 63°C and 60 min, respectively. Nested PCR was performed targeting the IS6110 region from M. tuberculosis using a commercial kit. The LAMP method yielded a sensitivity of 88.23% and a specificity of 80%, compared to the nested-PCR assay, which yielded a sensitivity of 52.9% and a specificity of 90% for TBM diagnosis. Comparative experiments showed that the LAMP assay is a rapid, sensitive, and specific method to detect TBM infection and that it is superior to the nested-PCR assay. LAMP is very simple, and it can be performed in any laboratory and in rural settings.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources.
“LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources (26–31).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [27]
The Introduction (page unavailable) says LAMP could be adopted by any laboratory, and the Discussion (page unavailable) describes its use for diagnosing tuberculous meningitis with an inexpensive turbidometer. This supports the LAMP example and suggests potential use in resource-limited laboratories, but the paper does not study NASBA or LAMP across various CNS infections. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805 · see citing works
Accelerated reaction by loop-mediated isothermal amplification using loop primers
Nagamine, K., Hase, T., & Notomi, T. (2002) · Molecular and Cellular Probes · doi:10.1006/mcpr.2002.0415 · Cited by 2,049 · see citing works
Mori, Y., Nagamine, K., Tomita, N., & Notomi, T. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.5921 · Cited by 1,715 · see citing works
Real-time turbidimetry of LAMP reaction for quantifying template DNA
Mori, Y., Kitao, M., Tomita, N., & Notomi, T. (2004) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2003.12.005 · Cited by 627 · see citing works
Iwamoto, T., Sonobe, T., & Hayashi, K. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.6.2616-2622.2003 · Cited by 617 · see citing works
Thai, H. T. C., Le, M. Q., Vuong, C. D., Parida, M., Minekawa, H., Notomi, T., Hasebe, F., & Morita, K. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.5.1956-1961.2004 · Cited by 423 · see citing works
Self-citationDiagnosis of adult tuberculous meningitis by use of clinical and laboratory features
Thwaites, G. E., Chau, T., Stepniewska, K., Phu, N. H., Chuong, L. V., Sinh, D. X. T., White, N. J., Parry, C., & Farrar, J. (2002) · The Lancet · doi:10.1016/s0140-6736(02)11318-3 · Cited by 396 · see citing works
Boehme, C. C., Nabeta, P., Henostroza, G., Raqib, R., Rahim, Z., Gerhardt, M., Sanga, E., Höelscher, M., Notomi, T., Hase, T., et al. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.02352-06 · Cited by 342 · see citing works
Han, E., Watanabe, R., Sattabongkot, J., Khuntirat, B., Sirichaisinthop, J., Iriko, H., Jin, L., Takeo, S., & Tsuboi, T. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.02117-06 · Cited by 277 · see citing works
Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., Mori, Y., & Yokomizo, Y. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.9.4359-4365.2003 · Cited by 166 · see citing works
Aryan, E., Makvandi, M., Sheikh, A. F., Huygen, K., Bifani, P. J., Gargari, S. L. M., Fateh, A., Jelodar, A., Gouya, M. M., & Romano, M. (2009) · Microbiological Research · doi:10.1016/j.micres.2009.05.001 · Cited by 153 · see citing works
Loop-Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
Pham, H. M., Nakajima, C., Ohashi, K., & Onuma, M. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.4.1646-1650.2005 · Cited by 134 · see citing works
Loop-Mediated Isothermal PCR (LAMP) for the Diagnosis of Falciparum Malaria
Paris, D. H., Imwong, M., Faiz, A. M., Hasan, M., Yunus, E. B., Silamut, K., Lee, S. J., Day, N. P. J., & Dondorp, A. M. (2007) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2007.77.972 · Cited by 132 · see citing works
Pandey, B. D., Poudel, A., Yoda, T., Tamaru, A., Oda, N., Fukushima, Y., Lekhak, B., Risal, B., Acharya, B., Sapkota, B. R., et al. (2008) · Journal of Medical Microbiology · doi:10.1099/jmm.0.47499-0 · Cited by 127 · see citing works
Parida, M. M., Santhosh, S. R., Dash, P. K., Tripathi, N. K., Saxena, P., Ambuj, S., Sahni, A. K., Lakshmana Rao, P. V., & Morita, K. (2006) · Journal of Clinical Microbiology · doi:10.1128/jcm.01487-06 · Cited by 120 · see citing works
Self-citationPathogenesis, diagnosis, treatment, and outcome aspects of cerebral tuberculosis.
Katti, M. K. (2004) · PubMed · Cited by 120 · see citing works
Okafuji, T., Yoshida, N., Fujino, M., Motegi, Y., Ihara, T., Ota, Y., Notomi, T., & Nakayama, T. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.4.1625-1631.2005 · Cited by 80 · see citing works
Dual-Probe Assay for Rapid Detection of Drug-Resistant Mycobacterium tuberculosis by Real-Time PCR
Wada, T., Maeda, S., Tamaru, A., Imai, S., Hase, A., & Kobayashi, K. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.11.5277-5285.2004 · Cited by 77 · see citing works
Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis
Deshpande, P. S., Kashyap, R. S., Ramteke, S. S., Nagdev, K. J., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2007) · Cerebrospinal Fluid Research · doi:10.1186/1743-8454-4-10 · Cited by 65 · see citing works
Self-citationZhu, R.-Y., Zhang, K., Zhao, M., Liu, Y., Xu, Y.-Y., Ju, C., Li, B., & Chen, J. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.07.006 · Cited by 60 · see citing works
Lee, M.-F., Chen, Y., & Peng, C. (2008) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2008.10.005 · Cited by 53 · see citing works
Kashyap, R. S., Dobos, K. M., Belisle, J. T., Purohit, H. J., Chandak, N. H., Taori, G. M., & Daginawala, H. F. (2005) · Clinical and Vaccine Immunology · doi:10.1128/cdli.12.6.752-758.2005 · Cited by 53 · see citing works
Self-citationPrognosis of tuberculous meningitis in adults in the era of modern antituberculous chemotherapy.
Wang, J., Hung, C., Sheng, W., Wang, J., Chang, S., & Luh, K. (2002) · PubMed · Cited by 48 · see citing works
Rafi, W., Mm, V., Nagarathna, S., Satishchandra, P., & Chandramuki, A. (2007) · PubMed · Cited by 44 · see citing works
Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis
Takahashi, T., Nakayama, T., Tamura, M., Ogawa, K., Tsuda, H., Morita, A., Hara, M., Togo, M., Shiota, H., Suzuki, Y., et al. (2005) · Neurology · doi:10.1212/01.wnl.0000162052.13838.b2 · Cited by 40 · see citing works
Venkataswamy, M., Rafi, W., Nagarathna, S., Ravi, V., & Chandramuki, A. (2007) · Indian Journal of Medical Microbiology · doi:10.4103/0255-0857.34765 · Cited by 36 · see citing works
Takahashi, T., Tamura, M., Asami, Y., Kitamura, E., Saito, K., Suzuki, T., Takahashi, S., Matsumoto, K., Sawada, S., Yokoyama, E., et al. (2008) · Journal of Clinical Microbiology · doi:10.1128/jcm.02214-07 · Cited by 34 · see citing works
Kashyap, R. S., Ramteke, S. P., Deshpande, P. S., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2007) · PubMed · Cited by 22 · see citing works
Self-citationAdhikari, B. R., Pandey, B. D., Ghimire, P., Shrestha, B., Khadka, M., Yoda, T., & Suzuki, Y. (2009) · Japanese Journal of Infectious Diseases · doi:10.7883/yoken.jjid.2009.212 · Cited by 20 · see citing works
Nagdev, K. J., Kashyap, R. S., Deshpande, P. S., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2010) · PubMed · Cited by 18 · see citing works
Self-citationZhang, J., Zhang, G., Yang, L., Huang, R., Zhang, Y., Jia, K., Yuan, W., & Li, S. (2010) · The Veterinary Journal · doi:10.1016/j.tvjl.2010.01.001 · Cited by 17 · see citing works
Murakami, S., Takeno, M., Oka, H., Ueda, A., Kurokawa, T., Kuroiwa, Y., & Ishigatsubo, Y. (2008) · Clinical and Vaccine Immunology · doi:10.1128/cvi.00029-08 · Cited by 16 · see citing works
Nagdev, K. J., Kashyap, R. S., Deshpande, P. S., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2010) · PubMed · Cited by 14 · see citing works
Self-citationPoudel, A., Pandey, B., Lekhak, B., Rijal, B. P., Sapkota, B., & Suzuki, Y. (1970) · Kathmandu University Medical Journal · doi:10.3126/kumj.v7i2.2701 · Cited by 11 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [5]5. Enosawa M., et al. 2003. Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 41:4359–4365 [DOI] [PMC free article] [PubMed] [Google Scholar]
Cited by
Sorted by citations
Acute bacterial meningitis in adults
McGill, F., Heyderman, R. S., Panagiotou, S., Tunkel, A. R., & Solomon, T. (2016) · The Lancet · doi:10.1016/s0140-6736(16)30654-7 · Cited by 274 · see citing works
A Versatile PDMS/Paper Hybrid Microfluidic Platform for Sensitive Infectious Disease Diagnosis
Dou, M., Domínguez, D. C., Li, X., Sanchez, J., & Scott, G. (2014) · Analytical Chemistry · doi:10.1021/ac5021694 · Cited by 205 · see citing works
Challenges and perspectives in the diagnosis of extrapulmonary tuberculosis
Norbis, L., Alagna, R., Tortoli, E., Codecasa, L. R., Migliori, G. B., & Cirillo, D. M. (2014) · Expert Review of Anti-infective Therapy · doi:10.1586/14787210.2014.899900 · Cited by 142 · see citing works
Manyelo, C. M., Solomons, R., Walzl, G., & Chegou, N. N. (2020) · Journal of Clinical Microbiology · doi:10.1128/jcm.01771-20 · Cited by 123 · see citing works
Zhou, D., Guo, J., Xu, L., Gao, S., Qingliang, L., Wu, Q., Wu, L., & Que, Y. (2014) · Scientific Reports · doi:10.1038/srep04912 · Cited by 110 · see citing works
Drobniewski, F. A., Cooke, M., Jordan, J., Casali, N., Mugwagwa, T., Broda, A., Townsend, C. L., Sivaramakrishnan, A., Green, N., Jit, M., et al. (2015) · Health Technology Assessment · doi:10.3310/hta19340 · Cited by 108 · see citing works
Methods of Rapid Diagnosis for the Etiology of Meningitis in Adults
Bahr, N. C., & Boulware, D. R. (2014) · Biomarkers in Medicine · doi:10.2217/bmm.14.67 · Cited by 108 · see citing works
Viral meningitis: current issues in diagnosis and treatment
McGill, F., Griffiths, M. J., & Solomon, T. (2017) · Current Opinion in Infectious Diseases · doi:10.1097/qco.0000000000000355 · Cited by 101 · see citing works
Blaser, S., Diem, H., von Felten, A., Gueuning, M., Andreou, M., Boonham, N., Tomlinson, J. A., Müller, P., Utzinger, J., Frey, J. E., et al. (2018) · Pest Management Science · doi:10.1002/ps.4866 · Cited by 78 · see citing works
Drobniewski, F. A., Nikolayevskyy, V., Balabanova, Y., Bang, D., & Papaventsis, D. C. (2012) · The International Journal of Tuberculosis and Lung Disease · doi:10.5588/ijtld.12.0180 · Cited by 76 · see citing works
Pormohammad, A., Nasiri, M. J., McHugh, T. D., Riahi, S. M., & Bahr, N. C. (2019) · Journal of Clinical Microbiology · doi:10.1128/jcm.01113-18 · Cited by 75 · see citing works
Drobniewski, F. A., Nikolayevskyy, V., Maxeiner, H., Balabanova, Y., Casali, N., Kontsevaya, I., & Ignatyeva, O. A. (2013) · BMC Medicine · doi:10.1186/1741-7015-11-190 · Cited by 72 · see citing works
Advances in the diagnosis and treatment of tuberculous meningitis
Thwaites, G. E. (2013) · Current Opinion in Neurology · doi:10.1097/wco.0b013e3283602814 · Cited by 71 · see citing works
Diagnosing tuberculous meningitis – have we made any progress?
Ho, J., Marais, B. J., Gilbert, G. L., & Ralph, A. P. (2013) · Tropical Medicine & International Health · doi:10.1111/tmi.12099 · Cited by 65 · see citing works
Serotyping dengue virus with isothermal amplification and a portable sequencer
Yamagishi, J., Runtuwene, L. R., Hayashida, K., Mongan, A. E., Thi, L. A. N., Thùy, L. N., Nhat, C. N., Limkittikul, K., Sirivichayakul, C., Sathirapongsasuti, N., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-03734-5 · Cited by 48 · see citing works
Sharma, K., Sharma, M., Batra, N., Sharma, A., & Dhillon, M. S. (2016) · Journal of Orthopaedic Research® · doi:10.1002/jor.23293 · Cited by 46 · see citing works
Li, J.-J., Xiong, C., Liu, Y., Liang, J., & Zhou, X. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01956 · Cited by 46 · see citing works
Yu, G., Shen, Y., Zhong, F., Ye, B., Yang, J., & Chen, G. (2018) · PLoS ONE · doi:10.1371/journal.pone.0199290 · Cited by 45 · see citing works
Zhang, Z., Liu, X., Li, D., Yu, J., & Han, C. (2011) · Virology Journal · doi:10.1186/1743-422x-8-550 · Cited by 44 · see citing works
Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38 · see citing works
Diagnosis of extra pulmonary tuberculosis: An update on novel diagnostic approaches
Jain, R., Gupta, G., Mitra, D. K., & Guleria, R. (2024) · Respiratory Medicine · doi:10.1016/j.rmed.2024.107601 · Cited by 38 · see citing works
Commercial nucleic acid amplification tests in tuberculous meningitis—a meta-analysis
Solomons, R., van Elsland, S. L., Visser, D. H., Hoek, K. G. P., Marais, B. J., Schoeman, J. F., & van Furth, A. M. (2014) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2014.01.002 · Cited by 36 · see citing works
Evaluation of improved IS6110 LAMP assay for diagnosis of pulmonary and extra pulmonary tuberculosis
Joon, D., Nimesh, M., Basil, M. V., & Saluja, D. (2017) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2017.05.007 · Cited by 35 · see citing works
Aryan, E., Makvandi, M., Farajzadeh, A., Huygen, K., Alvandi, A., Gouya, M. M., Sadrizadeh, A., & Romano, M. (2013) · Journal of Infection · doi:10.1016/j.jinf.2013.02.005 · Cited by 33 · see citing works
Sethi, S. K., Dhaliwal, L., Dey, P., Kaur, H., Yadav, R., & Sethi, S. K. (2016) · Indian Journal of Medical Microbiology · doi:10.4103/0255-0857.188323 · Cited by 33 · see citing works
Su, Y., Yang, Y., Peng, Q., Zhou, D., Chen, Y., Wang, Z., Xu, L., & Que, Y. (2016) · Scientific Reports · doi:10.1038/srep23994 · Cited by 33 · see citing works
Vibha, D., Bhatia, R., Prasad, K., Srivastava, M. V. P., Tripathi, M., Kumar, G., & Singh, M. B. (2012) · Clinical Neurology and Neurosurgery · doi:10.1016/j.clineuro.2011.12.033 · Cited by 30 · see citing works
Li, J., Zhai, L., Bie, X., Lu, Z., Kong, X., Yu, Q., Lv, F., Zhang, C., & Zhao, H. (2015) · Food Control · doi:10.1016/j.foodcont.2015.07.036 · Cited by 30 · see citing works
Improving Technology to Diagnose Tuberculous Meningitis: Are We There Yet?
Ssebambulidde, K., Gakuru, J., Ellis, J. P., Cresswell, F. V., & Bahr, N. C. (2022) · Frontiers in Neurology · doi:10.3389/fneur.2022.892224 · Cited by 30 · see citing works
Zhou, S., Wang, M., Liu, M., Jiang, K., Wang, B., & Wang, L. (2020) · Aquaculture · doi:10.1016/j.aquaculture.2020.734987 · Cited by 28 · see citing works
Yang, B., Wang, X., Li, H., Li, G., Cao, Z., & Cheng, X. (2011) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2011.03141.x · Cited by 28 · see citing works
Sharma, M., Sharma, K., Sharma, A., Gupta, N., & Rajwanshi, A. (2016) · Tuberculosis · doi:10.1016/j.tube.2016.07.015 · Cited by 28 · see citing works
Soo, P., Tseng, C., Ling, S.-R., Liou, M., Liu, C., Chao, H.-J., Lin, T., & Chang, K. (2012) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2012.11.020 · Cited by 27 · see citing works
Kumari, S., Kumar, R. R., Mendiratta, S. K., Kumar, D., Rana, P., Kumar, D., & Jawla, J. (2019) · 3 Biotech · doi:10.1007/s13205-019-1595-x · Cited by 26 · see citing works
Sun, W., Sun, Q., Yan, L., & Zhang, Q. (2017) · Oncotarget · doi:10.18632/oncotarget.15734 · Cited by 23 · see citing works
Kooti, S., Kadivarian, S., Abiri, R., Mohajeri, P., Atashi, S., Yazdi, H. A., & Alvandi, A. (2023) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-023-03564-w · Cited by 22 · see citing works
Patel, J. S., Brennan, M. S., Khan, A., & Ali, G. S. (2014) · Canadian Journal of Plant Pathology · doi:10.1080/07060661.2014.996610 · Cited by 22 · see citing works
Advances in tuberculous meningitis diagnosis
Ahlawat, S., Chaudhary, R., Dangi, M., Bala, K., Singh, M., & Chhillar, A. K. (2020) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2020.1858805 · Cited by 20 · see citing works
Yan, L., Peng, S. Z., Yan, P., Zhou, J., Teng, Q., Li, G., Li, X., & Li, Z. (2012) · Journal of Virological Methods · doi:10.1016/j.jviromet.2012.03.007 · Cited by 20 · see citing works
Arutselvan, R., Reddy, M. K., & Makeshkumar, T. (2017) · VirusDisease · doi:10.1007/s13337-017-0385-5 · Cited by 18 · see citing works
IS1081-based Multi-targeted LAMP: An Opportunity to Detect Tubercular Uveitis
Sharma, M., Singh, R., Sharma, A., Gupta, V., & Sharma, K. (2020) · Ocular Immunology and Inflammation · doi:10.1080/09273948.2020.1787462 · Cited by 17 · see citing works
Ramya, M., Kayalvizhi, M., Haripriya, G., & Rathinasabapathi, P. (2018) · 3 Biotech · doi:10.1007/s13205-018-1402-0 · Cited by 16 · see citing works
Sreedeep, K. S., Sethi, S. K., Yadav, R., Vaidya, P. C., Angurana, S. K., Saini, A., Mehra, N., & Singh, M. (2020) · Journal of Infection and Chemotherapy · doi:10.1016/j.jiac.2020.03.019 · Cited by 16 · see citing works
Kamra, E., Singh, N., Khan, A., Singh, J. K., Chauhan, M. B., Kamal, H., & Mehta, P. K. (2022) · BioTechniques · doi:10.2144/btn-2022-0027 · Cited by 15 · see citing works
Hou, J., Wu, H., Zeng, X. Z., Hui, R., & Zhao, P. (2018) · Medicine · doi:10.1097/md.0000000000013660 · Cited by 15 · see citing works
Cao, W.-F., Leng, E.-L., Liu, S., Zhou, Y.-L., Luo, C.-Q., Xiang, Z.-B., Cai, W., Rao, W., Hu, F., Zhang, P., et al. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1202752 · Cited by 13 · see citing works
Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents
Yoshikawa, T., Matsuo, T., Kawamura, Y., Ohashi, M., Yonekawa, T., Kanda, H., Notomi, T., & Ihira, M. (2014) · Journal of Virological Methods · doi:10.1016/j.jviromet.2014.02.017 · Cited by 13 · see citing works
HIV-Tuberculous Meningitis Co-Infection: A Systematic Review and Meta-Analysis
Pormohammad, A., Azimi, T., Nasiri, M. J., Goudarzi, M., Zangiabadian, M., Sedighian, H., & Fooladi, A. A. I. (2020) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201021666200730143906 · Cited by 13 · see citing works
Rajput, R., Singh, P., Sarin, R., Sethi, P., & Sharma, S. (2019) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2019.01.016 · Cited by 13 · see citing works
Khumwan, P., Pengpanich, S., Kampeera, J., Kamsong, W., Karuwan, C., Sappat, A., Srilohasin, P., Chaiprasert, A., Tuantranont, A., & Kiatpathomchai, W. (2022) · Microchemical Journal · doi:10.1016/j.microc.2021.107108 · Cited by 12 · see citing works
Mor, P., Sheoran, A., Dahiya, B., Parshad, S., Nain, R., Khan, A., Malhotra, P., Gulati, P., & Mehta, P. K. (2022) · Journal of Gastroenterology and Hepatology · doi:10.1111/jgh.16036 · Cited by 12 · see citing works
The Lab Without Walls: A Deployable Approach to Tropical Infectious Diseases
Inglis, T. J. J. (2013) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.12-0704 · Cited by 12 · see citing works
Kamala, S., & Makeshkumar, T. (2015) · Journal of Virological Methods · doi:10.1016/j.jviromet.2015.06.008 · Cited by 11 · see citing works
Nasarudin, S. N. S., Zainudin, N. S., Bernadus, M., Nawi, A. M., Hanafiah, A., & Osman, E. (2015) · Journal of Medical Microbiology · doi:10.1099/jmm.0.000156 · Cited by 11 · see citing works
Nagai, Y., Iwade, Y., Nakano, M., Akachi, S., Kobayashi, T., & Nishinaka, T. (2016) · Microbiology and Immunology · doi:10.1111/1348-0421.12389 · Cited by 11 · see citing works
Gupta, R., Talwar, P., Talwar, P., Khurana, S., Kushwaha, S. S., Jalan, N., & Thakur, R. (2018) · Journal of Infection · doi:10.1016/j.jinf.2018.04.017 · Cited by 11 · see citing works
Current Insights into Diagnosing and Treating Neurotuberculosis in Adults
Dian, S., Ganiem, A. R., Brake, L. H. M. T., & van Laarhoven, A. (2023) · CNS Drugs · doi:10.1007/s40263-023-01047-y · Cited by 9 · see citing works
Molecular diagnostic methods for rapid diagnosis of central nervous system infections
Mallikarjuna, P. V., Upadhya, D., Yegneswaran, P. P., Varghese, A., Pahadasingh, S., Prabhu, A., Saravu, K., & Shettigar, K. S. (2025) · Frontiers in Medical Technology · doi:10.3389/fmedt.2025.1497512 · Cited by 10 · see citing works
Loop-Mediated Isothermal Amplification for Rapid Diagnosis of Tubercular Uveitis—Reply
Sharma, K., Sharma, A., & Gupta, A. (2014) · JAMA Ophthalmology · doi:10.1001/jamaophthalmol.2014.4261 · Cited by 9 · see citing works
Sharma, K., Sharma, M., Modi, M., Goyal, M., Sharma, A., & Ray, P. (2020) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2020.101595 · Cited by 8 · see citing works
Singh, P., Kanade, S. R., & Nataraj, G. (2021) · International Journal of Mycobacteriology · doi:10.4103/ijmy.ijmy_218_21 · Cited by 8 · see citing works
Bumbrah, G. S., Jain, S., Fatima, Z., & Hameed, S. (2023) · Drug Target Insights · doi:10.33393/dti.2023.2596 · Cited by 7 · see citing works
Advancing Diagnosis and Treatment in People Living with HIV and Tuberculosis Meningitis
Kimuda, S., Kasozi, D., Namombwe, S., Gakuru, J., Mugabi, T., Kagimu, E., Rutakingirwa, M. K., Leon, K. E., Chow, F. C., Wasserman, S. A., et al. (2023) · Current HIV/AIDS Reports · doi:10.1007/s11904-023-00678-6 · Cited by 7 · see citing works
Guo, Y.-L., Wang, S., Jiang, G., Ban, W.-J., & Liu, Y. (2022) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2022.106547 · Cited by 6 · see citing works
Addo, S. O., Abrahams, A. O. D., Mensah, G. I., Mawuli, B. A., Mosi, L., Wiredu, E. K., & Addo, K. K. (2022) · Heliyon · doi:10.1016/j.heliyon.2022.e12370 · Cited by 5 · see citing works
Neurotuberculosis and HIV Infection
Alexandra, S., & Gabriela, D. (2013) · InTech eBooks · doi:10.5772/54631 · Cited by 5 · see citing works
Li, J., Du, J., Li, S. H., Dong, J., Ying, J., Gu, Y., Lü, J., Xinyu, Z., Kear, P. J., Dou, D., et al. (2023) · Phytopathology Research · doi:10.1186/s42483-023-00179-9 · Cited by 4 · see citing works
Mittal, V., Kumar, M., & Singh, A. K. (2024) · The Journal of Infection in Developing Countries · doi:10.3855/jidc.18115 · Cited by 4 · see citing works
Laboratory Diagnosis of Tuberculosis - Latest Diagnostic Tools
Şenol, G. (2013) · InTech eBooks · doi:10.5772/54248 · Cited by 4 · see citing works
Low-complexity manual nucleic acid amplification tests for pulmonary tuberculosis in children
Inbaraj, L. R., Narayanan, M. K. S., Daniel, J., Srinivasalu, V. A., Bhaskar, A., Daniel, B. D., Epsibha, T., Scandrett, K., Rajendran, P., Rose, W., et al. (2025) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd015806.pub2 · Cited by 3 · see citing works
Tuberculous meningitis in Asia
Feng, G., Zhang, L., & Zhao, G. (2015) · Cited by 3 · see citing works
Mitha, M., Pillay, M., Moodley, J., Balakrishna, Y., Abbai, N. S., Bhagwan, S., Dangor, Z., & Bhigjee, A. I. (2020) · Southern African Journal of Infectious Diseases · doi:10.4102/sajid.v35i1.135 · Cited by 3 · see citing works
Manke, S. (2017) · JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH · doi:10.7860/jcdr/2017/24493.9668 · Cited by 2 · see citing works
Tuberculosis in Mine Workers: Advances in Current Diagnostic Landscape
Husain, A. A., Nayak, A. R., Jain, R. K., Daginawala, H. F., Tumane, R., Jawade, A. A., Pingle, S., & Kashyap, R. S. (2022) · Springer geology · doi:10.1007/978-3-030-99495-2_3 · Cited by 2 · see citing works
Self-citationEvaluation of Loop-Mediated Isothermal Amplification Assay in Diagnosing Tuberculous Meningitis
Chauhan, V. S., Jorwal, P., Singh, B. K., Nischal, N., Soneja, M., Vyas, S., Vishnu, V. Y., Sethi, P., Ranjan, P., & Wig, N. (2024) · Cureus · doi:10.7759/cureus.57490 · Cited by 2 · see citing works
Forbes, B. A. (2016) · Springer eBooks · doi:10.1007/978-3-319-19674-9_53 · Cited by 2 · see citing works
Li, Z., Chen, S., Wang, S., Chen, S., & Lin, F. (2012) · Folia Microbiologica · doi:10.1007/s12223-012-0204-8 · Cited by 1 · see citing works
Kumar, K. V., & Kandula, M. (2012) · Journal of AIDS & Clinical Research · doi:10.4172/2155-6113.1000154 · Cited by 1 · see citing works
Times have Changed! The Molecular Revolution in TB Diagnostics and Detection of Drug Resistance
Koornhof, H. J., & Coetzee, G. J. (2011) · Southern African Journal of Epidemiology and Infection · doi:10.1080/10158782.2011.11441458
Evaluation of tuberculosis meningitis within the last twenty years in Iran
Varshochi, M., Naghili, B., & Zarrintan, V. (2013)
Arifin, M. I. (2016) · Epsilon Archive for Student Projects (University of Southampton)
Li, J., Du, J., Li, S. H., Dong, J., Ying, J., Gu, Y., Lu, J., Xinyu, Z., Kear, P. J., Dou, D., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26588994
Li, J., Du, J., Li, S. H., Dong, J., Ying, J., Gu, Y., Lu, J., Xinyu, Z., Kear, P. J., Dou, D., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26588991
Wu, J., Zhao, Z., Pang, P., Tie, Y., Jiao, S., Zhang, D., Liu, X., Wang, J., Wang, Y., Tan, H., et al. (2026) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2026.1781397
Treatment and Challenges of Tubercular Meningitis
Dhamija, K., & Netravathi, M. (2026) · Springer eBooks · doi:10.1007/978-981-92-0256-0_12
- Authors
- Nagdev, K. J., Kashyap, R. S., Parida, M. M., Kapgate, R. C., Purohit, H. J., Taori, G. M., & Daginawala, H. F.
- Affiliations
- Central India Institute of Medical Sciences (IN)
- Defence Research and Development Establishment (IN)
- National Environmental Engineering Research Institute (IN)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-05
- Volume
- 49
- Issue
- 5
- Pages
- 1861-1865
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC3122663
- OpenAlex
- W2167033417
- Cited by
- 86
- References
- 34
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 1 · 2020: 8 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 10 · 2015: 5 · 2014: 8 · 2013: 8 · 2012: 6
- FWCI
- 4.73
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Keywords
- tuberculous meningitis, loop-mediated isothermal amplification, Mycobacterium tuberculosis, IS6110, nested PCR, rapid molecular diagnostics, sensitivity and specificity, point-of-care testing
- MeSH terms
- Adolescent, Adult, Aged, Bacteriological Techniques, Child, DNA Transposable Elements, DNA, Bacterial, Female, Humans, Male, Middle Aged, Mycobacterium tuberculosis, Sensitivity and Specificity, Temperature, Tuberculosis, Meningeal, DNA Primers, Nucleic Acid Amplification Techniques, Molecular Diagnostic Techniques, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
