The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci
Open accessAbstract
In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease ( nuc ) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus , S. hyicus , S. schleiferi , S. intermedius , S. pseudintermedius , and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.
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Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals
Devriese, L. A., Vancanneyt, M., Baele, M., Vaneechoutte, M., De Graef, E., Snauwaert, C., Cleenwerck, I., Dawyndt, P., Swings, J., Decostere, A., et al. (2005) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijs.0.63413-0 · Cited by 374
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Cited by
Himsworth, C. G., Miller, R. R., Montoya, V., Hoang, L., Romney, M. G., Al-Rawahi, G. N., Kerr, T., Jardine, C. M., Patrick, D. M., Tang, P., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0087983 · Cited by 60
“The lukF-PV gene encoding the PantonValentine leukocidin toxin was detected by real-time PCR [17].”
MethodInfluentialMethicillin-Resistant Staphylococcus pseudintermedius in Rats
Himsworth, C. G., Patrick, D. M., Parsons, K., Feng, A., & Weese, J. S. (2013) · Emerging infectious diseases · doi:10.3201/eid1901.120897 · Cited by 14
Metadata
- Authors
- Sasaki, T., Tsubakishita, S., Tanaka, Y., Sakusabe, A., Ohtsuka, M., Hirotaki, S., Kawakami, T., Fukata, T., & Hiramatsu, K.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-03
- Volume
- 48
- Issue
- 3
- Pages
- 765-769
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2156668469
- Cited by
- 438
- References
- 22
- FWCI
- 12.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Sasaki, T., Tsubakishita, S., Tanaka, Y., Sakusabe, A., Ohtsuka, M., Hirotaki, S., Kawakami, T., Fukata, T., & Hiramatsu, K. (2010). Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.01232-09
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