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Cited by 184

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Comparative Performance of Five Commercially Available Serologic Assays To Detect Antibodies to SARS-CoV-2 and Identify Individuals with High Neutralizing Titers

Open access
Patel, Eshan U., Bloch, Evan M., Clarke, William, Hsieh, Yu-Hsiang, Boon, Denali, Eby, Yolanda, Fernandez, Reinaldo E., Baker, Owen R., Keruly, Morgan, Kirby, Charles S., Klock, Ethan, Littlefield, Kirsten, Miller, Jernelle, Schmidt, Haley A., Sullivan, Philip, Piwowar-Manning, Estelle, Shrestha, Ruchee, Redd, Andrew D., Rothman, Richard E., Sullivan, David, Shoham, Shmuel, Casadevall, Arturo, Quinn, Thomas C., Pekosz, Andrew, Tobian, Aaron A. R., Laeyendecker, Oliver·2021·Journal of Clinical Microbiology

Abstract

Accurate serological assays to detect antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to characterize the epidemiology of SARS-CoV-2 infection and identify potential candidates for coronavirus disease 2019 (COVID-19) convalescent plasma (CCP) donation. This study compared the performances of commercial enzyme immunoassays (EIAs) with respect to detection of IgG or total antibodies to SARS-CoV-2 and neutralizing antibodies (nAbs). The diagnostic accuracy of five commercially available EIAs (Abbott, Euroimmun, EDI, ImmunoDiagnostics, and Roche) for detection of IgG or total antibodies to SARS-CoV-2 was evaluated using cross-sectional samples from potential CCP donors who had prior molecular confirmation of SARS-CoV-2 infection ( n = 214) and samples from prepandemic emergency department patients without SARS-CoV-2 infection ( n = 1,099).

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Metadata

Authors
Patel, E. U., Bloch, E. M., Clarke, W., Hsieh, Y.-H., Boon, D., Eby, Y., Fernandez, R. E., Baker, O. R., Keruly, M., Kirby, C. S., Klock, E., Littlefield, K., Miller, J., Schmidt, H. A., Sullivan, P., Piwowar-Manning, E., Shrestha, R., Redd, A. D., Rothman, R. E., Sullivan, D., Shoham, S., Casadevall, A., Quinn, T. C., Pekosz, A., Tobian, A. A. R., & Laeyendecker, O.
Venue
Journal of Clinical Microbiology
Published
2021-01-21
Volume
59
Issue
2
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W3097263615
Cited by
184
References
1
FWCI
5.07
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Comparative Performance of Five Commercially Available Serologic Assays To Detect Antibodies to SARS-CoV-2 and Identify Individuals with High Neutralizing Titers. https://app.cochat.ai/papers/10.1128/jcm.02257-20
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Patel, E. U., Bloch, E. M., Clarke, W., Hsieh, Y.-H., Boon, D., Eby, Y., Fernandez, R. E., Baker, O. R., Keruly, M., Kirby, C. S., Klock, E., Littlefield, K., Miller, J., Schmidt, H. A., Sullivan, P., Piwowar-Manning, E., Shrestha, R., Redd, A. D., Rothman, R. E., . . . Laeyendecker, O. (2021). Comparative Performance of Five Commercially Available Serologic Assays To Detect Antibodies to SARS-CoV-2 and Identify Individuals with High Neutralizing Titers. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.02257-20

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.