Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.
Open accessAbstract
Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.
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Asymptomatic sexually abused children might be infected with T. vaginalis and might benefit from treatment.
“Testing for T. vaginalis should not be limited to girls with vaginal discharge if other indications for vaginal testing exist because evidence indicates that asymptomatic sexually abused children might be infected with T. vaginalis and might benefit from treatment (1451,1452).”
From Sexually Transmitted Infections Treatment Guidelines, 2021 (2021) · cites this paper as [1451]
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- Authors
- Bandea, C. I., Joseph, K., Secor, E. W., Jones, L. A., Igietseme, J. U., Sautter, R. L., Hammerschlag, M. R., Fajman, N. N., Girardet, R. G., & Black, C. M.
- Affiliations
- Centers for Disease Control and Prevention (US)
- Carolinas Pathology Group, Charlotte, North Carolina, USA
- SUNY Downstate Health Sciences University (US)
- Emory University (US)
- The University of Texas Health Science Center at Houston (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2013-04
- Volume
- 51
- Issue
- 4
- Pages
- 1298-1300
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC3666790
- OpenAlex
- W2019072047
- Cited by
- 30
- References
- 27
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 1
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- SUNY Digital Repository Support (State University of New York System) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Trichomonas vaginalis, trichomoniasis, PCR detection, urine samples, non-invasive diagnosis, protozoan parasites, diagnostic test development
- MeSH terms
- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Parasitology, Sensitivity and Specificity, Trichomonas Infections, Trichomonas vaginalis, Urine, Polymerase Chain Reaction, Clinical Laboratory Techniques, Molecular Diagnostic Techniques
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