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Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids

Open access
Miller, L T·1982·Journal of Clinical Microbiology

Abstract

Analysis of fatty acid methyl esters prepared from whole-cell bacteria by sodium hydroxide hydrolysis and boron trichloride-catalyzed methylation showed degradation of hydroxy acid peaks after several injections on a fused silica capillary column. A simple base wash of the extracts before injection prevented the tailing of the hydroxy acid peaks even after extended use. This eliminates the need to form trifluoroacetic anhydride derivatives of the hydroxy acids.

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Crossref · 10.1128/jcm.16.3.584-586.1982

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Metadata

Authors
Miller, L. T.
Venue
Journal of Clinical Microbiology
Published
1982-09
Volume
16
Issue
3
Pages
584-586
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W1528590781
Cited by
1,100
References
0
FWCI
2.03
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. https://app.cochat.ai/papers/10.1128/jcm.16.3.584-586.1982
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Miller, L. T. (1982). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.16.3.584-586.1982

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.