Infection of peripheral blood mononuclear cells and cell lines by cell-free human T-cell lymphoma/leukemia virus type I
Open accessAbstract
Previous studies of in vitro infection by human T-cell lymphoma/leukemia virus type I (HTLV-I) have required cocultivation of target cells with HTLV-I cell lines or vesicular stomatitis virus pseudotypes containing HTLV-I envelope proteins. We report here the development of a cell-free infection assay for HTLV-I. Target cells were incubated with purified, DNase-treated HTLV-I virions for 4 h at 37 degrees C. Target cell DNA was then analyzed for the presence of newly synthesized HTLV-I proviral DNA by the highly sensitive polymerase chain reaction. Using this assay system, we have been able to consistently detect in vitro infection of a variety of cellular targets by different HTLV-I isolates. Optimal infection required the presence of 10 micrograms of DEAE-dextran per ml. The assay was dose dependent with respect to virus input. In general, the amount of proviral DNA detected correlated with the level of HTLV-I receptors present on the surface of the target cells, as measured by fluorochrome-labelled HTLV-I binding. Finally, the specificity of the assay was confirmed by demonstrating that the cell line, L1q, a somatic cell hybrid containing human chromosome 17q, to which the gene for the HTLV-I receptor has been mapped, was susceptible to infection by HTLV-I, while the parental mouse cell line from which it was derived, LMTK-, which lacks human chromosome 17q, was not.
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HTLV-1-infected T cells produce very few free virions in vitro, of which only 1 out of 10^5-10^6 is infectious.
“HTLV-1-infected T cells have been shown to produce very few free virions in vitro, of which only 1 out of 105–106 is infectious [20,21].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [21]
In the “Effects of DEAE-dextran” section (page not available), the paper estimates roughly one infectious particle per 10^6 HTLV-I virions in its cell-free infection assay, supporting the infectivity figure. The Methods describe obtaining virions from infected cell cultures, but do not establish that infected T cells produce very few free virions in vitro. (LLM, conf=0.87)
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Cell-free HTLV-1 infects dendritic cells leading to transmission and transformation of CD4+ T cells
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HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
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“HTLV-1-infected T cells have been shown to produce very few free virions in vitro, of which only 1 out of 105–106 is infectious [20,21].”
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- Authors
- Fan, N., Gavalchin, J., Paul, B., Wells, K. H., Lane, M. J., & Poiesz, B. J.
- Affiliations
- SUNY Upstate Medical University (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1992-04
- Volume
- 30
- Issue
- 4
- Pages
- 905-910
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC265183
- OpenAlex
- W1843784436
- Cited by
- 131
- References
- 32
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 1 · 2012: 4
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hg000318-03, 5r01hl043602-03)
- National Heart, Lung, and Blood Institute (R01 HL-43602-01)
- National Human Genome Research Institute (HG00318)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Keywords
- HTLV-1, peripheral blood mononuclear cells, HTLV-I proviral DNA, DEAE-dextran, chromosome 17q, HTLV-1 infection
- MeSH terms
- Adult, Cell Line, DNA, Viral, Humans, Kinetics, Leukocytes, Mononuclear, Proviruses, Viral Proteins, Virology, Human T-lymphotropic virus 1, HTLV-I Infections, Polymerase Chain Reaction, In Vitro Techniques
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