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Cited by 211

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

Open access
Rotbart, H A, Sawyer, M H, Fast, S, Lewinski, C, Murphy, N, Keyser, E F, Spadoro, J, Kao, S Y, Loeffelholz, M·1994·Journal of Clinical Microbiology

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

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Crossref · 10.1128/jcm.32.10.2590-2592.1994

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Metadata

Authors
Rotbart, H. A., Sawyer, M. H., Fast, S., Lewinski, C., Murphy, N., Keyser, E. F., Spadoro, J., Kao, S. Y., & Loeffelholz, M.
Venue
Journal of Clinical Microbiology
Published
1994-10
Volume
32
Issue
10
Pages
2590-2592
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W1634669351
Cited by
211
References
1
FWCI
6.29
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. https://app.cochat.ai/papers/10.1128/jcm.32.10.2590-2592.1994
The paper
Rotbart, H. A., Sawyer, M. H., Fast, S., Lewinski, C., Murphy, N., Keyser, E. F., Spadoro, J., Kao, S. Y., & Loeffelholz, M. (1994). Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.32.10.2590-2592.1994

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.