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Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR
Open accessAbstract
A PCR assay that allows simultaneous detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci (Enterococcus faecium, E. faecalis, E. gallinarum, and E. casseliflavus) was developed. This assay was based on specific amplification of internal fragments of genes encoding D-alanine:D-alanine ligases and related glycopeptide resistance proteins. The specificity of the assay was tested on 5 well-characterized glycopeptide-resistant strains and on 15 susceptible enterococcal type strains. Clinical isolates of enterococci that could not be identified to the species level by conventional methods were identified by the PCR test. This assay offers a specific and rapid alternative to antibiotic susceptibility tests, in particular for detection of low-level vancomycin resistance.
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Metadata
- Authors
- Dutka-Malen, S., Evers, S., & Courvalin, P.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-01
- Volume
- 33
- Issue
- 1
- Pages
- 24-27
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1961220478
- Cited by
- 1,403
- References
- 0
- FWCI
- 16.39
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Dutka-Malen, S., Evers, S., & Courvalin, P. (1995). Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.33.1.24-27.1995
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
