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Cited by 60Bacterial Identification and Susceptibility TestingBacterial Infections and VaccinesPneumonia and Respiratory Infections

PCR-Enzyme Immunoassay for Detection of Streptococcus pneumoniae DNA in Cerebrospinal Fluid Samples from Patients With Culture-Negative Meningitis

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Cherian, Thomas, Lalitha, M. K., Manoharan, Anand, Thomas, Kurien, Yolken, Robert H., Steinhoff, Mark C.·1998·Journal of Clinical Microbiology
DOI 10.1128/jcm.36.12.3605-3608.1998PMC PMC105248
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Abstract

A PCR-based assay was developed to amplify a conserved region of the pneumococcal autolysin gene. The amplified product was labelled with digoxigenin-labelled dUTP and was detected with a biotin-labelled probe in an enzyme immunoassay (EIA). The assay was initially tested with suspensions of various serotypes of Streptococcus pneumoniae and other gram-positive and gram-negative bacteria and was then applied to cerebrospinal fluid (CSF) specimens from patients with meningitis and those with other neurological disorders. The assay detected all the serotypes of S. pneumoniae tested, whereas all the other bacterial strains tested were negative. Seven of the 8 CSF specimens positive for pneumococcus by culture or latex agglutination (LA) were positive by PCR-EIA, whereas all 10 specimens positive for other organisms were negative. Among 11 patients with clinically diagnosed meningitis but with negative culture and LA results, 5 were positive by PCR-EIA. The assay was negative for all but one patient without meningitis; it was positive with the CSF from a child with immunodeficiency and pneumococcal abscesses on the scalp. PCR-EIA is a useful tool for the diagnosis of meningitis, especially when culture and LA are negative because of prior antibiotic treatment.

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  • The reported sensitivity of PCR for pneumococcal meningitis is high and reached 100% in the majority of studies, with a median of 100% and range 79%-100%.

    “The reported sensitivity of PCR for pneumococcal meningitis is high and reached 100% in the majority of conducted studies (median of 100%, range 79%–100%) (Table 1; see also Table S1 in the supplemental material) (49–58).”

    From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [52]

    The Discussion (p. 3) reports that PCR-EIA detected pneumococcus in 7 of 8 culture- or latex-agglutination-positive CSF specimens, supporting a high detection rate in this study. The paper does not report that a majority of studies reached 100% sensitivity or establish the cited median and range across studies. (LLM, conf=0.85)

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Authors
Cherian, T., Lalitha, M. K., Manoharan, A., Thomas, K., Yolken, R. H., & Steinhoff, M. C.
Affiliations
  • <!--label omitted: 1-->Departments of Child Health,1
  • <!--label omitted: 2-->Clinical Microbiology,2 and
  • Christian Medical College, Vellore (IN)
  • <!--label omitted: 4-->Pediatrics4 and
  • Johns Hopkins University (US)
ORCID
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
1998-12
Volume
36
Issue
12
Pages
3605-3608
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.36.12.3605-3608.1998
PMC
PMC105248
OpenAlex
W2118120153
Cited by
60
References
21
Citations by year
2024: 1 · 2022: 1 · 2020: 1 · 2019: 2 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 2 · 2012: 3
FWCI
2.00
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
Keywords
Streptococcus pneumoniae, culture-negative meningitis, cerebrospinal fluid, molecular diagnosis, serotyping, latex agglutination test, prior antibiotic use, immunodeficiency
MeSH terms
Child, Child, Preschool, DNA, Bacterial, Humans, Immunoenzyme Techniques, Infant, Infant, Newborn, Meningitis, Pneumococcal, Sensitivity and Specificity, Streptococcus pneumoniae, Polymerase Chain Reaction

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