The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus
Open accessAbstract
Staphylococcus aureus is the cause of serious infections in humans, including endocarditis, deep-seated abscesses, and bacteremia, which lead to toxic and septic shock syndromes. Rapid and direct identification of this bacterium specifically and ubiquitously directly from clinical specimens would be useful in improving the diagnosis of S. aureus infections in the clinical microbiology laboratory. A wide variety of kits based on biochemical characteristics efficiently identify S. aureus, but the rapidity and the accuracy of each of these methods combined with testing of clinically relevant antibiotic resistance genes need to be improved. On the basis of hybridization assays with randomly selected clones from an S. aureus genomic library, we have identified a chromosomal DNA fragment which is specific for S. aureus and which detected all 82 S. aureus isolates tested. This 442-bp fragment was sequenced and was used to design a set of PCR amplification primers. The PCR assay was also specific and ubiquitous for the identification from bacterial cultures of 195 clinical strains of S. aureus isolated from a variety of anatomical sites and obtained from hospitals throughout the world. The PCR assay that we have developed is simple and can be performed in about 1 h. This DNA-based test provides a novel diagnostic tool for the diagnosis of S. aureus infections.
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Cites
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Cited by
Kateete, D. P., Kimani, C. N., Katabazi, F. A., Okeng, A., Okee, M. S., Nanteza, A., Joloba, M. L., & Najjuka, F. C. (2010) · Annals of Clinical Microbiology and Antimicrobials · doi:10.1186/1476-0711-9-23 · Cited by 359
rpoB Gene Sequence-Based Identification of Staphylococcu s Species
Drancourt, M., & Raoult, D. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.4.1333-1338.2002 · Cited by 309
Costa, A.-M., Kay, I., & Palladino, S. (2005) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2004.08.014 · Cited by 117
Occurrence of methicillin-resistant Staphylococcus aureus in rats living on pig farms
van de Giessen, A. W., van Santen-Verheuvel, M. G., Hengeveld, P. D., Bosch, T., Broens, E. M., & Reusken, C. B. E. M. (2009) · Preventive Veterinary Medicine · doi:10.1016/j.prevetmed.2009.05.016 · Cited by 91
Food-Animal Related Staphylococcus aureus Multidrug-Resistant ST9 Strains with Toxin Genes
He, W., Liu, Y., Qi, J., Chen, H., Zhao, C., Zhang, F., Li, H., & Wang, H. (2013) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2012.1452 · Cited by 25
Metadata
- Authors
- Martineau, F., Picard, F. J., Roy, P. H., Ouellette, M., & Bergeron, M. G.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-03
- Volume
- 36
- Issue
- 3
- Pages
- 618-623
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2139378390
- Cited by
- 494
- References
- 31
- FWCI
- 9.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Martineau, F., Picard, F. J., Roy, P. H., Ouellette, M., & Bergeron, M. G. (1998). Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.36.3.618-623.1998
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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