Development of a Plasmodium PCR for Monitoring Efficacy of Antimalarial Treatment
Open accessAbstract
We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium -specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high specificity for the four human Plasmodium species, and we were able to detect one parasite in 50 μl of whole blood. The responses of 12 patients infected with Plasmodium falciparum to antimalarial therapy were monitored by PCR diagnosis and examination of thick blood film for at least 20 min by an experienced microscopist. For one patient this study allowed early diagnosis of therapeutic failure, confirmed 7 days later by examination of the thick blood film. A total of 134 samples were examined; 94 were positive by PCR, and among these 68 were positive by thick blood film examination. The sensitivity of the thick blood film was 72.3% compared to PCR and 60.7% compared to dot blot hybridization.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Most published PCR assays are gel based with or without subsequent probe hybridizations.
“However, most published PCR assays are gel based with (4, 5, 15) or without (2, 3, 9, 23, 25-27, 29, 31, 35, 36) subsequent probe hybridizations”
From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [5]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Bottius, E., Guanzirolli, A., Trape, J.-F., Rogier, C., Konate, L., & Druilhe, P. (1996) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/s0035-9203(96)90463-0 · Cited by 190 · see citing works
Barker, R. H., Banchongaksorn, T., Courval, J. M., Suwonkerd, W., Rimwungtragoon, K., & Wirth, D. F. (1992) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1992.46.416 · Cited by 148 · cited 1× · see citing works
“The first studies of nucleic acidbased malaria detection used the repetitive genomic DNA as a target (3, 22).”
Background
Flagged first, then by citations
Research works printed but not linked: 3
- [6]6. Craig, M. H., and B. L. Sharp. 1997. Comparative evaluation of four techniques for the diagnosis of Plasmodium falciparum infections. Trans. R. Soc. Trop. Med. Hyg. 91: 279-282.
- [7]7. Gay, F., B. Traore, J. Zanoni, M. Danis, and A. Fribourg-Blanc. 1996. Direct acridine orange fluorescence examination of blood slides compared to current techniques for malaria diagnosis. Trans. R. Soc. Trop. Med. Hyg. 90: 516-518.
- [25]25. Vu, T. T., V. B. Tran, N. T. Phan, T. T. Le, V. H. Luong, E. O'Brien, and G. E. Morris. 1995. Screening donor blood for malaria by polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg. 89: 44-47.
Cited by
Sorted by citations
Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278 · see citing works
- Authors
- Ciceron, L., Jaureguiberry, G., Gay, F., & Danis, M.
- Affiliations
- <!--label omitted: 1-->Service de Parasitologie et UnitéINSERM 313, Centre Hospitalier-Universitaire Pitié-Salpêtrière, 75013 Paris,1 and
- <!--label omitted: 2-->EP 1790 CNRS, Laboratoire de Biologie Parasitaire, MNHN, 75005 Paris,2 France
- Inserm (FR)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-01
- Volume
- 37
- Issue
- 1
- Pages
- 35-38
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC84160
- OpenAlex
- W2144432722
- Cited by
- 59
- References
- 2
- Citations by year
- 2023: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Plasmodium species detection, malaria, small subunit rRNA gene, Plasmodium falciparum, PCR diagnosis, thick blood films, dot blot, treatment failure, parasite detection sensitivity
- MeSH terms
- Animals, Antimalarials, Humans, Plasmodium, Plasmodium falciparum, RNA, Ribosomal, 18S, Sensitivity and Specificity, DNA, Protozoan, Polymerase Chain Reaction, Malaria, Falciparum, Process Assessment, Health Care
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
