The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Direct Amplification of rRNA Genes in Diagnosis of Bacterial Infections
Open accessAbstract
A broad-range bacterial PCR targeting rRNA genes (rDNAs) was used to directly analyze 536 clinical samples obtained from 459 hospitalized patients during a 4-year study period. The molecular diagnosis based on DNA sequencing of the PCR product was compared to that obtained by bacterial culture. The bacteriological diagnosis was concordant for 447 (83%) specimens. Broad-range rDNA PCR was the only method that yielded an etiologic diagnosis for 11 (2.4%) of 459 patients. Compared to culture and clinical assessment, the sensitivity of the PCR method combined with sequencing was 74.2%, and the specificity was between 98.7 and 99.6%. At present, the described molecular approach proved superior to bacterial culture in two clinical situations: infections caused by bacteria with unusual growth requirements and specimens taken during antimicrobial treatment of the patient.
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Woese, C. R. (1987) · Microbiological Reviews · doi:10.1128/mr.51.2.221-271.1987 · Cited by 5,042
The Agent of Bacillary Angiomatosis: An Approach to the Identification of Uncultured Pathogens
Relman, D. A., Loutit, J. S., Schmidt, T. M., Falkow, S., & Tompkins, L. S. (1990) · New England Journal of Medicine · doi:10.1056/nejm199012063232301 · Cited by 901
Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing
Goldenberger, D., Künzli, A., Vogt, P., Zbinden, R., & Altwegg, M. (1997) · Journal of Clinical Microbiology · doi:10.1128/jcm.35.11.2733-2739.1997 · Cited by 390
Cited by
Schuurman, T., de Boer, R. F., Kooistra-Smid, A. M. D., & van Zwet, A. A. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.2.734-740.2004 · Cited by 234
Microbial diversity in chronic open wounds
Frank, D. N., Wysocki, A., Specht‐Glick, D. D., Rooney, A., Feldman, R. A., St. Amand, A. L., Pace, N. R., & Trent, J. D. (2009) · Wound Repair and Regeneration · doi:10.1111/j.1524-475x.2009.00472.x · Cited by 123
Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis
(2003) · Emerging Infectious Diseases · doi:10.3201/eid0912.030229 · Cited by 79
Metadata
- Authors
- Rantakokko-Jalava, K., Nikkari, S., Jalava, J., Eerola, E., Skurnik, M., Meurman, O., Ruuskanen, O., Alanen, A., Kotilainen, E., Toivanen, P., & Kotilainen, P.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-01
- Volume
- 38
- Issue
- 1
- Pages
- 32-39
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1652636050
- Cited by
- 217
- References
- 3
- FWCI
- 7.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Rantakokko-Jalava, K., Nikkari, S., Jalava, J., Eerola, E., Skurnik, M., Meurman, O., Ruuskanen, O., Alanen, A., Kotilainen, E., Toivanen, P., & Kotilainen, P. (2000). Direct Amplification of rRNA Genes in Diagnosis of Bacterial Infections. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.38.1.32-39.2000
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