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Identification of the Causative Organism of Tuberculous Lymphadenitis in Ethiopia by PCR
Open accessAbstract
Tuberculous lymphadenitis (TBLN) is a common form of extrapulmonary tuberculosis with multiple differential diagnoses. Demonstration of the etiologic agent by smear microscopy or culture of fine needle aspirate (FNA) specimens is often unsuccessful. FNA specimens from 40 patients presenting at a rural health center in South Ethiopia and diagnosed as positive for TBLN on the basis of clinical and cytological criteria were analyzed for mycobacterial DNA by PCR. Thirty (75%) had cervical lymphadenitis and 11 (27.5%) were seropositive for human immunodeficiency virus (HIV). Three primer sets were initially used to identify the causative agent at the genus (antigen 85 complex), complex (IS 6110 insertion sequence), and species ( pncA gene and allelic variation) levels. Among the forty TBLN cases, 35 (87.5%) were positive by PCR at the genus and complex levels. Based on PCR for detection of allelic variation at position 169, 24 (68.6%) of the 35 were positive for Mycobacterium tuberculosis and 6 (17.1%) were positive for M. bovis . These six were positive in additional PCR assays using the JB21-JB22 primer set, which is highly specific for M. bovis. Five (14.1%) showed amplification for both M. tuberculosis and M. bovis with the allele-specific primer set . Cooccurrence of pyrazinamide (PZA)-sensitive and -resistant M. tuberculosis in those five cases was indicated, since all were negative in assays with the JB21-JB22 primer set. This feature was seen in 3 of 11 HIV-positive and 2 of 29 HIV-negative individuals ( P < 0.001). Conclusion: among 35 PCR-positive cases of TBLN from southern Ethiopia, 29 (82.9%) were caused by M. tuberculosis and six (17.1%) were caused by M. bovis.
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“PCR sensitivity was found to be 83% and specificity to be 100% in diagnosing TBLN with FNAC [32].”
From Comparative Diagnostic of Cervical Tuberculous Lymphadenitis: PCR is a Fast, Efficient, and Improved Diagnostic Approach (2023) · cites this paper as [32]
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Self-citation
Metadata
- Authors
- Kidane, D., Olobo, J. O., Habte, A., Negesse, Y., Aseffa, A., Abate, G., Yassin, M. A., Bereda, K., & Harboe, M.
- Affiliations
- Armauer Hansen Research Institute (ET)
- Southern Nations, Nationalities, and Peoples' Region (ET)
- Butajira Health Center, Southern Region, Butajira District, Ethiopia
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2002-11
- Volume
- 40
- Issue
- 11
- Pages
- 4230-4234
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC139683
- OpenAlex
- W2097086169
- Cited by
- 126
- References
- 26
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 6 · 2017: 3 · 2016: 4 · 2015: 13 · 2014: 13 · 2013: 8 · 2012: 3
- FWCI
- 3.53
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Styrelsen för Internationellt Utvecklingssamarbete
- Armauer Hansen Research Institute
- Direktoratet for Utviklingssamarbeid
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Infectious Diseases › Diagnosis and treatment of tuberculosis
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- tuberculous lymphadenitis, extrapulmonary tuberculosis, Mycobacterium tuberculosis, Mycobacterium bovis, molecular identification, pncA, IS6110 insertion sequence, antigen 85 complex, pyrazinamide resistance, HIV coinfection, cervical lymphadenitis
- MeSH terms
- Biopsy, Needle, DNA, Bacterial, Ethiopia, Humans, Mycobacterium bovis, Mycobacterium tuberculosis, Sensitivity and Specificity, Tuberculosis, Lymph Node, Polymerase Chain Reaction, DNA Primers
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