The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Quantitative Multiprobe PCR Assay for Simultaneous Detection and Identification to Species Level of Bacterial Pathogens
Open accessAbstract
We describe a novel adaptation of the TaqMan PCR assay which potentially allows for highly sensitive detection of any eubacterial species with simultaneous species identification. Our system relies on a unique multiprobe design in which a single set of highly conserved sequences encoded by the 16S rRNA gene serves as the primer pair and is used in combination with both an internal highly conserved sequence, the universal probe, and an internal variable region, the species-specific probe. A pre-PCR ultrafiltration step effectively decontaminates or removes background DNA. The TaqMan system described reliabAly detected 14 common bacterial species with a detection limit of 50 fg. Further, highly sensitive and specific pathogen detection was demonstrated with a prototype species-specific probe designed to detect Staphylococcus aureus . This assay has broad potential in the clinical arena for rapid and specific diagnosis of infectious diseases.
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Cites
Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing
Goldenberger, D., Künzli, A., Vogt, P., Zbinden, R., & Altwegg, M. (1997) · Journal of Clinical Microbiology · doi:10.1128/jcm.35.11.2733-2739.1997 · Cited by 390
Cited by
Simon, M.-C., Strassburger, K., Nowotny, B., Kolb, H., Nowotny, P., Burkart, V., Zivehe, F., Hwang, J.-H., Stehle, P., Pacini, G., et al. (2015) · Diabetes Care · doi:10.2337/dc14-2690 · Cited by 253
Enrichment of oral microbiota in early cystic precursors to invasive pancreatic cancer
Gaiser, R. A., Halimi, A., Alkharaan, H., Lu, L., Davanian, H., Healy, K., Hugerth, L. W., Ateeb, Z., Valente, R., Fernández Moro, C., et al. (2019) · Gut · doi:10.1136/gutjnl-2018-317458 · Cited by 227
Kortman, G. A. M., Mulder, M. L. M., Richters, T. J. W., Shanmugam, N. K. N., Trebicka, E., Boekhorst, J., Timmerman, H. M., Roelofs, R., Wiegerinck, E. T., Laarakkers, C. M., et al. (2015) · European Journal of Immunology · doi:10.1002/eji.201545642 · Cited by 65
Iwai, S., Huang, D., Fong, S., Jarlsberg, L. G., Worodria, W., Yoo, S., Cattamanchi, A., Davis, J. L., Kaswabuli, S., Segal, M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0095726 · Cited by 58
Metadata
- Authors
- Yang, S., Lin, S., Kelen, G. D., Quinn, T. C., Dick, J. D., Gaydos, C. A., & Rothman, R. E.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2002-09
- Volume
- 40
- Issue
- 9
- Pages
- 3449-3454
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2145233046
- Cited by
- 218
- References
- 1
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Yang, S., Lin, S., Kelen, G. D., Quinn, T. C., Dick, J. D., Gaydos, C. A., & Rothman, R. E. (2002). Quantitative Multiprobe PCR Assay for Simultaneous Detection and Identification to Species Level of Bacterial Pathogens. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.40.9.3449-3454.2002
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