The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Development and Evaluation of a Seminested PCR for Detection and Differentiation of Babesia gibsoni(Asian Genotype) and B. canis DNA in Canine Blood Samples
Open accessAbstract
Canine babesiosis has recently been recognized as an emerging infectious disease of dogs in North America. We sought to develop a seminested PCR to detect and differentiate Babesia gibsoni(Asian genotype),B. canis subsp.vogeli,B. canis subsp.canis, and B. canis subsp.rossi DNA in canine blood samples. An outer primer pair was designed to amplify an ∼340-bp fragment of the 18S rRNA genes from B. gibsoni(Asian genotype),B. canis subsp.vogeli,B. canis subsp.rossi, and B. canis subsp.canis but not mammalian DNA. Forward primers were designed that would specifically amplify a smaller fragment from each organism in a seminested PCR. The practical limit of detection was 50 organisms/ml of mock-infected EDTA anticoagulated whole blood. The primer pair also amplified an ∼370-bp fragment of the B. gibsoni(USA/California genotype) 18S rRNA gene from the blood of an experimentally infected dog with a high percentage of parasitemia. Amplicons were not detected when DNA extracted from the blood of a dog that was naturally infected with Theileria annae at a low percentage of parasitemia was amplified. Due to limited sensitivity, this test is not recommended for the routine diagnosis of B. gibsoni(USA/California genotype) or T. annae. The PCR test did not amplify Toxoplasma gondii,Neospora caninum,Leishmania infantum,Cryptosporidium parvum, or canine DNA under any of the conditions tested. The seminested PCR test was able to detect and discriminate B. gibsoni(Asian genotype),B. canis subsp.vogeli,B. canis subsp.canis, and B. canis subsp.rossi DNA in blood samples from infected dogs.
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Cites
Benson, D. A. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.1.17 · Cited by 455
Detection of a new pathogenic Babesia microti-like species in dogs
Zahler, M., Rinder, H., Schein, E., & Gothe, R. (2000) · Veterinary Parasitology · doi:10.1016/s0304-4017(00)00202-8 · Cited by 260
Three groups of Babesia canis distinguished and a proposal for nomenclature
Uilenberg, G., Franssen, F. F. J., Perié, N. M., & Spanjer, A. A. M. (1989) · Veterinary Quarterly · doi:10.1080/01652176.1989.9694194 · Cited by 235
Cacciò, S. M., Antunovic, B., Moretti, A., Mangili, V., Marinculic, A., Baric, R. R., Slemenda, S. B., & Pieniazek, N. J. (2002) · Veterinary Parasitology · doi:10.1016/s0304-4017(02)00112-7 · Cited by 191
Zahler, M., Schein, E., Rinder, H., & Gothe, R. (1998) · Parasitology Research · doi:10.1007/s004360050445 · Cited by 186
CARRET, C., WALAS, F., CARCY, B., GRANDE, N., PRÉCIGOUT, É., MOUBRI, K., SCHETTERS, T. P., & GORENFLOT, A. (1999) · Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.1999.tb05128.x · Cited by 182
There are at least three genetically distinct small piroplasms from dogs
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Infection of dogs in north‐west Spain with a Babesia microti ‐like agent
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Babesia gibsoni infections in dogs from North Carolina
Birkenheuer, A. J., Levy, M. G., Savary, K. C. M., Gager, R. B., & Breitschwerdt, E. B. (1999) · Journal of the American Animal Hospital Association · doi:10.5326/15473317-35-2-125 · Cited by 118
Self-citationBabesia gibsoni infection among dogs in the southeastern United States
Macintire, D. K., Boudreaux, M. K., West, G. D., Bourne, C., Wright, J. C., & Conrad, P. A. (2002) · Journal of the American Veterinary Medical Association · doi:10.2460/javma.2002.220.325 · Cited by 112
Cited by
Babesiosis in dogs and cats—Expanding parasitological and clinical spectra
Solano-Gallego, L., & Baneth, G. (2011) · Veterinary Parasitology · doi:10.1016/j.vetpar.2011.04.023 · Cited by 334
Hamel, D., Silaghi, C., Lescai, D., & Pfister, K. (2012) · Parasitology Research · doi:10.1007/s00436-011-2659-y · Cited by 82
Pantchev, N., Pluta, S., Huisinga, E., Nather, S., Scheufelen, M., Vrhovec, M. G., Schweinitz, A., Hampel, H., & Straubinger, R. K. (2015) · Parasitology Research · doi:10.1007/s00436-015-4513-0 · Cited by 68
Hamel, D., Silaghi, C., Zapadynska, S., Kudrin, A., & Pfister, K. (2013) · Ticks and Tick-borne Diseases · doi:10.1016/j.ttbdis.2012.08.005 · Cited by 48
(2009) · PubMed · Cited by 47
Metadata
- Authors
- Birkenheuer, A. J., Levy, M. G., & Breitschwerdt, E. B.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-09
- Volume
- 41
- Issue
- 9
- Pages
- 4172-4177
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2117089016
- Cited by
- 549
- References
- 13
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- green
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