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Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1
Open accessAbstract
We constructed lambda recombinants containing the Harvey murine sarcoma virus genome and the thymidine kinase (tk) gene of herpes simplex virus type 1 linked to each other. The tk gene was located in a position downstream from both the long terminal repeat and the src gene of Harvey murine sarcoma virus. The DNAs of the lambda recombinants were used to transfect NIH3T3 mouse fibroblasts in order to obtain Harvey murine sarcoma virus DNA-induced foci of transformed cells. The transformed foci were superinfected with a helper-independent retrovirus, and new individual retrovirus were isolated from the superinfected foci. The new viruses could induce focus formation on NIH3T3 cells and could convert NIH3T3(TK-) cells into TK+ cells by carrying the herpes simplex virus type 1 tk gene into the TK- cells. From virus-infected cells, we isolated nonproducer foci on NIH3T3 cells and TK+ transformants on NIH3T3(TK-) cells containing one such new viral genome coding for the dual properties. The new retroviral sequence in the nonproducer cells could be rescued into virus particles at high titers by superinfection with a helper-independent retrovirus. A hybridization analysis indicated that the recombinant virus contained both the Harvey murine sarcoma virus src sequence and the tk gene sequence in a single RNA species approximately 4.9 kilobases long. We concluded that retroviruses can be used as true vectors for genes other than genes that lead to oncogenesis.
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Cites
DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA
STEHELIN, D., VARMUS, H. E., BISHOP, J. M., & VOGT, P. K. (1976) · Nature · doi:10.1038/260170a0 · Cited by 1,321
Differences between the Ribonucleic Acids of Transforming and Nontransforming Avian Tumor Viruses
Duesberg, P. H., & Vogt, P. K. (1970) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.67.4.1673 · Cited by 269
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Kavanaugh, M. P., Miller, D. G., Zhang, W., Law, W., Kozak, S. L., Kabat, D., & Miller, A. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7071 · Cited by 586
Benns, J. M., Choi, J.-S., Mahato, R. I., Park, J.-S., & Kim, S. W. (2000) · Bioconjugate Chemistry · doi:10.1021/bc0000177 · Cited by 381
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Smith, G. L., & Moss, B. (1983) · Gene · doi:10.1016/0378-1119(83)90163-4 · Cited by 324
Metadata
- Authors
- Wei, C. M., Gibson, M., Spear, P. G., & Scolnick, E. M.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1981-09
- Volume
- 39
- Issue
- 3
- Pages
- 935-944
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1595240710
- Cited by
- 239
- References
- 2
- FWCI
- 11.31
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wei, C. M., Gibson, M., Spear, P. G., & Scolnick, E. M. (1981). Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. Journal of Virology. https://doi.org/10.1128/jvi.39.3.935-944.1981
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