The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
pH-Sensitive Cationic Polymer Gene Delivery Vehicle: N-Ac-poly(l-histidine)-graft-poly(l-lysine) Comb Shaped Polymer
Abstract
Advancing biotechnology spurs the development of new pharmaceutically engineered gene delivery vehicles. Poly(L-histidine) ¿PLH¿ has been shown to induce membrane fusion at endosomal pH values, whereas PLL has a well documented efficacy in polyplex formation. Therefore, N-Ac-poly(L-histidine)-graft-poly(L-lysine) ¿PLH-g-PLL¿ was synthesized by grafting poly(L-histidine) to poly(L-lysine) ¿PLL¿. PLH-g-PLL formed polyplex particles by electrostatic interactions with plasmid DNA ¿pDNA¿. The mean particle size of the polyplexes was in the range of 117 +/- 6 nm to 306 +/- 77 nm. PLH-g-PLL gene carrier demonstrated higher transfection efficacy in 293T cells than PLL at all equivalent weight ratios with pDNA. The inclusion of chloroquine as an endosomolytic agent enhanced transfection for both PLL and PLH-g-PLL gene carriers. PLH-g-PLL enhanced beta-galactosidase expression compared to PLL, but still increased in efficacy when chloroquine was included.
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Cites
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Cited by
Stimuli-reponsive polymers and their bioconjugates
GIL, E., & HUDSON, S. (2004) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2004.08.003 · Cited by 2,385
Nonviral vectors for gene delivery
Mintzer, M. A., & Simanek, E. E. (2009) · Chemical Reviews · doi:10.1021/cr800409e · Cited by 2,366
Prospects for cationic polymers in gene and oligonucleotide therapy against cancer
Merdan, T., Kopec̆ek, J., & Kissel, T. (2002) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00046-7 · Cited by 785
Smart polymeric gels: Redefining the limits of biomedical devices
Chaterji, S., Kwon, I. K., & Park, K. (2007) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2007.05.018 · Cited by 615
Ren, X., Feng, Y., Guo, J., Wang, H., Li, Q., Yang, J., Hao, X., Lv, J., Ma, N., & Li, W. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00483c · Cited by 573
Metadata
- Authors
- Benns, J. M., Choi, J.-S., Mahato, R. I., Park, J.-S., & Kim, S. W.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-09-01
- Volume
- 11
- Issue
- 5
- Pages
- 637-645
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W1997664422
- Cited by
- 381
- References
- 37
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- closed
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- Benns, J. M., Choi, J.-S., Mahato, R. I., Park, J.-S., & Kim, S. W. (2000). pH-Sensitive Cationic Polymer Gene Delivery Vehicle: N-Ac-poly(l-histidine)-graft-poly(l-lysine) Comb Shaped Polymer. Bioconjugate Chemistry. https://doi.org/10.1021/bc0000177
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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