The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses
Open accessAbstract
Thirteen clones of hybrid cells which synthesize antibodies directed against the Rous sarcoma virus (RSV) transforming protein, pp60src, were isolated. Mouse myeloma cells were fused with spleen cells from mice that had been immunized with purified pp60src from bacterial recombinants which direct the synthesis of the RSV src gene. The hybridomas which survived the selection medium were screened by immunoprecipitation of pp60src from 32P-labeled lysates of RSV-transformed cells. Monoclonal antibodies produced by subclones derived from 13 hybridomas recognized pp60src encoded by the Schmidt-Ruppin and Prague strains of RSV and the cellular homolog of pp60src. Antibody from clone 261 had a high affinity for the viral yes gene product, and antibodies from clones 443 and 463 recognized the transforming proteins encoded by viruses containing the related transforming genes fps and ros. Several other clones had a low affinity for the viral yes, fps, and ros gene products which could be detected by in vitro phosphorylation of the transforming proteins after immunoprecipitation with the monoclonal antibody. All of the monoclonal antibodies allowed phosphorylation of pp60src and casein in an immune complex-bound reaction.
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Cites
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Transforming gene product of Rous sarcoma virus phosphorylates tyrosine
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DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA
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Protein kinase activity associated with the avian sarcoma virus src gene product.
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Identification of a transformation-specific antigen induced by an avian sarcoma virus
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Self-citationLevinson, A. D., Oppermann, H., Levintow, L., Varmus, H. E., & Bishop, J. M. (1978) · Cell · doi:10.1016/0092-8674(78)90024-7 · Cited by 653
Vinculin: A cytoskeletal target of the transforming protein of rous sarcoma virus
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Cited by
Enhanced DNA-Binding Activity of a Stat3-Related Protein in Cells Transformed by the Src Oncoprotein
Yu, C.-L., Meyer, D. J., Campbell, G. S., Larner, A. C., Carter-Su, C., Schwartz, J., & Jove, R. (1995) · Science · doi:10.1126/science.7541555 · Cited by 931
Heat-shock protein hsp90 governs the activity of pp60v-src kinase.
Xu, Y., & Lindquist, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.15.7074 · Cited by 416
Huang, M. M., Bolen, J. B., Barnwell, J. W., Shattil, S. J., & Brugge, J. S. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.17.7844 · Cited by 406
Self-citationIncrease in activity and level of pp60c-src in progressive stages of human colorectal cancer.
Talamonti, M. S., Roh, M. S., Curley, S. A., & Gallick, G. E. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116200 · Cited by 399
Reynolds, A. B., Roesel, D. J., Kanner, S. B., & Parsons, J. T. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.2.629 · Cited by 342
Metadata
- Authors
- Lipsich, L. A., Lewis, A. J., & Brugge, J. S.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1983-11
- Volume
- 48
- Issue
- 2
- Pages
- 352-360
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1660489924
- Cited by
- 494
- References
- 24
- FWCI
- 10.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Lipsich, L. A., Lewis, A. J., & Brugge, J. S. (1983). Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses. Journal of Virology. https://doi.org/10.1128/jvi.48.2.352-360.1983
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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