The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220
Open accessAbstract
Poliovirus infection of HeLa cells induces rapid shutoff of host protein synthesis, whereas translation of poliovirus RNA is not inhibited. It is presumed that shutoff is the result of proteolytic cleavage of component p220 of eucaryotic initiation factor 4F. To study whether poliovirus proteinase 2A is involved in this cleavage, we translated synthetic RNAs that contained the coding region for poliovirus-specific polypeptides P1 and 2A in vitro and assayed for cleavage of p220. We report here that cleavage of p220 occurred in all cases when active proteinase 2A was translated and that disruption of the coding sequence of 2A by linker insertion or deletion prevented processing of p220 in vitro. Activity of 2A was determined by its ability to cleave at the P1-P2 site of a segment of the poliovirus polyprotein. We also constructed a plasmid in which the 3'-most 500 nucleotides of the nontranslated region of encephalomyocarditis virus were linked to the coding sequence for poliovirus polypeptide 2A. Translation of the RNA transcript of this clone was very efficient and yielded a fusion protein that included 2A; this polypeptide also induced cleavage of p220. In vitro translation in the presence of antibodies against 2A specifically inhibited processing of p220, whereas incubation of in vitro translation products with antibodies against 2A after translation was completed did not prevent proteolysis of p220.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Appleyard, R. K. (1954) · Genetics · doi:10.1093/genetics/39.4.440 · Cited by 955
Primary structure, gene organization and polypeptide expression of poliovirus RNA
Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., et al. (1981) · Nature · doi:10.1038/291547a0 · Cited by 914
Self-citationSmith, D. (1984) · The Journal of Cell Biology · doi:10.1083/jcb.99.1.20 · Cited by 660
Etchison, D., Milburn, S. C., Edery, I., Sonenberg, N., & Hershey, J. W. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33352-0 · Cited by 583
Self-citationJackson, R. J., & Hunt, T. (1983) · Methods in Enzymology · doi:10.1016/s0076-6879(83)96008-1 · Cited by 551
Cited by
Jang, S. K., Kräusslich, H. G., Nicklin, M. J., Duke, G. M., Palmenberg, A. C., & Wimmer, E. (1988) · Journal of Virology · doi:10.1128/jvi.62.8.2636-2643.1988 · Cited by 1,362
Self-citationInitiation of Protein Synthesis in Eukaryotic Cells
Pain, V. M. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.00747.x · Cited by 707
Antisense RNA: Function and Fate of Duplex RNA in Cells of Higher Eukaryotes
Kumar, M., & Carmichael, G. G. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.4.1415-1434.1998 · Cited by 320
Joachims, M., Van Breugel, P. C., & Lloyd, R. E. (1999) · Journal of Virology · doi:10.1128/jvi.73.1.718-727.1999 · Cited by 269
RNA-protein interactions in regulation of picornavirus RNA translation
Belsham, G. J., & Sonenberg, N. (1996) · Microbiological Reviews · doi:10.1128/mmbr.60.3.499-511.1996 · Cited by 266
Metadata
- Authors
- Kräusslich, H. G., Nicklin, M. J., Toyoda, H., Etchison, D., & Wimmer, E.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1987-09
- Volume
- 61
- Issue
- 9
- Pages
- 2711-2718
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2122966339
- Cited by
- 320
- References
- 37
- FWCI
- 13.20
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kräusslich, H. G., Nicklin, M. J., Toyoda, H., Etchison, D., & Wimmer, E. (1987). Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220. Journal of Virology. https://doi.org/10.1128/jvi.61.9.2711-2718.1987
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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