The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen
Open accessAbstract
Beginning with three partial cDNA clones of the RNA genome of human hepatitis delta virus (HDV), we assembled the complete 1,679-base sequence on a single molecule and then inserted a trimer of this into plasmid pSLV, a simian virus 40-based eucaryotic expression vector. This construct was used to transfect both monkey kidney (COS7) and human hepatocellular carcinoma (HuH7) cell lines. In this way we obtained replication of the HDV RNA genome and the appearance, in the nucleoli, of the delta antigen, the only known virus-coded protein. This proved both that the HDV genome could replicate in nonliver as well as liver cells and that there was no requirement for the presence of hepatitis B virus sequences or proteins. When the pSVL construct was made with a dimer of an HDV sequence with a 2-base-pair deletion in the open reading frame, genome replication was reduced at least 40-fold. However, when we cotransfected with a plasmid that expressed the correct delta antigen, the mutated dimer achieved a level of genome replication comparable to that of the nonmutated sequence. We thus conclude that the delta antigen can act in trans and is essential for replication of the HDV genome.
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Cites
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Cited by
A 'unified theory' of prion propagation
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Metadata
- Authors
- Kuo, M. Y., Chao, M., & Taylor, J.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-05
- Volume
- 63
- Issue
- 5
- Pages
- 1945-1950
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1495704598
- Cited by
- 447
- References
- 20
- FWCI
- 19.58
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kuo, M. Y., Chao, M., & Taylor, J. (1989). Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. Journal of Virology. https://doi.org/10.1128/jvi.63.5.1945-1950.1989
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
