The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein
Open accessAbstract
We have performed a detailed analysis of the biochemical properties of the human immunodeficiency virus (HIV) type 1 vpu gene product to elucidate its function during virus replication. Our data suggest that vpu is posttranslationally modified by phosphorylation, since a 16-kilodalton phosphoprotein can be specifically immunoprecipitated with both a serum from an HIV-positive individual (HIV-positive serum) and a vpu-specific antiserum. In contrast, our results suggest that vpu is not glycosylated, even though the protein contains a potential glycosylation site. In vitro translation studies demonstrated that vpu is cotranslationally integrated into microsomal membranes, suggesting that vpu is an integral membrane protein. While vpu was found in significant quantities in virus-producing cells, the protein could not be detected in cell-free culture fluids and is therefore most likely not viron associated. Processing of viral precursor proteins was unaffected by the absence of vpu, and no differences were detected in the protein compositions of wild-type and mutant virions. However, virus release from cultures producing vpu-defective virus was found to be delayed, resulting in the intracellular accumulation of viral proteins. Our data suggest that vpu has a function in the release of virus particles from infected cells.
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Cites
A new technique for the assay of infectivity of human adenovirus 5 DNA
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Self-citationGorman, C. M., Merlino, G. T., Willingham, M. C., Pastan, I., & Howard, B. H. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.22.6777 · Cited by 1,298
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Self-citationWilley, R. L., Smith, D. H., Lasky, L. A., Theodore, T. S., Earl, P. L., Moss, B., Capon, D. J., & Martin, M. A. (1988) · Journal of Virology · doi:10.1128/jvi.62.1.139-147.1988 · Cited by 751
Self-citation[6] Preparation of microsomal membranes for cotranslational protein translocation
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Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface
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A Novel Gene of HIV-1, vpu , and Its 16-Kilodalton Product
Strebel, K., Klimkait, T., & Martin, M. A. (1988) · Science · doi:10.1126/science.3261888 · Cited by 447
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Cited by
Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4
Willey, R. L., Maldarelli, F., Martin, M. A., & Strebel, K. (1992) · Journal of Virology · doi:10.1128/jvi.66.12.7193-7200.1992 · Cited by 554
Self-citationRücker, E., Grivel, J.-C., Münch, J., Kirchhoff, F., & Margolis, L. (2004) · Journal of Virology · doi:10.1128/jvi.78.22.12689-12693.2004 · Cited by 48
The translational landscape of HIV-1 infected cells reveals key gene regulatory principles
Kibe, A., Buck, S., Gribling-Burrer, A.-S., Gilmer, O., Bohn, P., Koch, T., Mireisz, C. N.-M., Schlosser, A., Erhard, F., Smyth, R. P., et al. (2025) · Nature Structural & Molecular Biology · doi:10.1038/s41594-024-01468-3 · Cited by 19
Metadata
- Authors
- Strebel, K., Klimkait, T., Maldarelli, F., & Martin, M. A.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-09
- Volume
- 63
- Issue
- 9
- Pages
- 3784-3791
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1555930389
- Cited by
- 275
- References
- 18
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Strebel, K., Klimkait, T., Maldarelli, F., & Martin, M. A. (1989). Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein. Journal of Virology. https://doi.org/10.1128/jvi.63.9.3784-3791.1989
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
