The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor
Open accessAbstract
The proteolytic processes involved in the cotranslational production of the Semliki Forest virus proteins p62, 6K, and E1 from a common precursor polypeptide were analyzed by an in vitro translation-translocation assay. By studying the behavior of wild-type and mutant variants of the polyprotein, we show that the signal sequences responsible for membrane translocation of the 6K and E1 proteins reside in the C-terminal regions of p62 and 6K, respectively. We present evidence suggesting that the polyprotein is processed on the luminal side by signal peptidase at consensus cleavage sites immediately following the signal sequences. Our results also lead us to conclude that the 6K protein is a transmembrane polypeptide with its N terminus on the luminal side of the membrane (type I). Thus, the production of all three membrane proteins is directed by alternating signal and stop-transfer (anchor) sequences that function in translocation and cleavage of the virus precursor polyprotein. This also shows conclusively that internally located signal sequences can be cleaved by signal peptidase.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
The alphaviruses: gene expression, replication, and evolution.
Strauss, J. H., & Strauss, E. G. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.3.491-562.1994 · Cited by 2,221
A comprehensive review of signal peptides: Structure, roles, and applications
Owji, H., Nezafat, N., Negahdaripour, M., Hajiebrahimi, A., & Ghasemi, Y. (2018) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2018.06.003 · Cited by 523
Gonzalez, M. E., & Carrasco, L. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00780-4 · Cited by 359
Semliki Forest Virus Expression System: Production of Conditionally Infectious Recombinant Particles
Berglund, P., Sjöberg, M., Garoff, H., Atkins, G. J., Sheahan, B. J., & Liljeström, P. (1993) · Nature Biotechnology · doi:10.1038/nbt0893-916 · Cited by 289
Self-citationSelf-replicating Semliki Forest virus RNA as recombinant vaccine
Zhou, X., Berglund, P., Rhodes, G., Parker, S. E., Jondal, M., & Liljeström, P. (1994) · Vaccine · doi:10.1016/0264-410x(94)90074-4 · Cited by 193
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Metadata
- Authors
- Liljeström, P., & Garoff, H.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1991-01
- Volume
- 65
- Issue
- 1
- Pages
- 147-154
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1518668585
- Cited by
- 220
- References
- 1
- FWCI
- 3.75
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor. https://app.cochat.ai/papers/10.1128/jvi.65.1.147-154.1991
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- Liljeström, P., & Garoff, H. (1991). Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor. Journal of Virology. https://doi.org/10.1128/jvi.65.1.147-154.1991
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
