Cited by 193Influenza Virus Research StudiesVirology and Viral DiseasesRNA Interference and Gene Delivery

Self-replicating Semliki Forest virus RNA as recombinant vaccine

Zhou, X, Berglund, P, Rhodes, G, Parker, S.E, Jondal, M, Liljeström, P·1994·Vaccine

Abstract

Recombinant RNA based on the Semliki Forest virus (SFV) replicon was used to express the nucleoprotein of influenza virus in mice. Two strategies were employed to deliver the RNA. In the first, recombinant RNA was packaged into infectious suicide SFV particles which were used directly for immunization. The second approach involved injection of in vitro-synthesized RNA directly into the quadriceps muscle. Both approaches resulted in the generation of humoral responses with high antibody titres. Immunization with suicide particles showed that a strong, class I-restricted cytotoxic T-cell response can be obtained using only 100 infectious units. We conclude that the self-replicative recombinant SFV RNA may be quite useful as a nucleic acid vaccine.

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Crossref · 10.1016/0264-410x(94)90074-4

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31 of 31 references verified0 citation errors · 0 not checkable

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  • The idea of using in vitro synthesized saRNA as a preventive vaccine was first proposed by Zhou and colleagues in 1994, using a modified Semliki Forest virus (SFV) replicon to express the nucleoprotein (NP) of the influenza virus.

    “The idea of using in vitro synthesized saRNA as a preventive vaccine was first proposed by Zhou and colleagues in 1994, using a modified Semliki Forest virus (SFV) replicon to express the nucleoprotein (NP) of the influenza virus.197”

    From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [197]

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Authors
Zhou, X., Berglund, P., Rhodes, G., Parker, S. E., Jondal, M., & Liljeström, P.
Affiliations
  • Karolinska Institutet (SE)
  • Vical (United States) (US)
Corresponding author
Liljeström, P
Venue
Vaccine
Publisher
Elsevier BV
Published
1994-12
Volume
12
Issue
16
Pages
1510-1514
ISSN
0264-410X (ISSN-L)
OpenAlex
W2006168695
Cited by
193
References
32
Citations by year
2026: 3 · 2025: 6 · 2024: 9 · 2023: 17 · 2022: 10 · 2021: 13 · 2020: 8 · 2019: 6 · 2018: 1 · 2017: 3 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 4
FWCI
6.37
Type
journal article
Language
en
Open access
closed
Locations
  • Vaccine (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
Semliki Forest virus, nucleic acid vaccines, influenza nucleoprotein, humoral immune response, cytotoxic T lymphocytes, self-replicating RNA
MeSH terms
Animals, Mice, Inbred BALB C, RNA, Viral, Semliki forest virus, T-Lymphocytes, Cytotoxic, Transfection, Vaccines, Synthetic, Viral Vaccines, Virus Replication, Mice

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