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Loss of p53 protein in human papillomavirus type 16 E6-immortalized human mammary epithelial cells
Abstract
We have shown previously that introduction of the human papillomavirus type 16 (HPV16) or HPV18 genome into human mammary epithelial cells induces their immortalization. These immortalized cells have reduced growth factor requirements. We report here that transfection with a single HPV16 gene E6 is sufficient to immortalize these cells and reduce their growth factor requirements. The RB protein is normal in these cells, but the p53 protein is sharply reduced, as shown by immunoprecipitation with anti-p53 antibody (pAB 421). We infer that the E6 protein reduces the p53 protein perhaps by signalling its destruction by the ubiquitin system. The HPV-transforming gene E7 was unable to immortalize human mammary epithelial cells. Thus, cell-specific factors may determine which viral oncogene plays a major role in oncogenesis.
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“co-transformation assays in murine kidney cells and human mammary epithelial cells established that HPV-16 E6 has transforming properties, inducing anchorage-independent growth and tumour formation in nude mice [95,96]; however, low-risk E6 were unable to do so [97]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [96]
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Cited by
Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells
Elenbaas, B., Spirio, L., Koerner, F., Fleming, M. D., Zimonjic, D. B., Donaher, J. L., Popescu, N. C., Hahn, W. C., & Weinberg, R. A. (2001) · Genes & Development · doi:10.1101/gad.828901 · Cited by 878
Interaction of Papillomavirus E6 Oncoproteins with a Putative Calcium-Binding Protein
Chen, J. J., Reid, C. E., Band, V., & Androphy, E. J. (1995) · Science · doi:10.1126/science.7624774 · Cited by 275
Self-citationBasukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Billecke, C. A., Ljungman, M. E., McKay, B. C., Rehemtulla, A., Taneja, N., & Ethier, S. P. (2002) · Oncogene · doi:10.1038/sj.onc.1205546 · Cited by 28
Metadata
- Authors
- Band, V, De Caprio, J. A., Delmolino, L., Kulesa, V, & Sager, R.
- Affiliations
- Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1991-12
- Volume
- 65
- Issue
- 12
- Pages
- 6671-6676
- ISSN
- 0022-538X
- 1098-5514
- Cited by
- 145
- References
- 2
- Language
- en
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