Cited by 72Viral Infections and Immunology ResearchVirology and Viral DiseasesEvolution and Genetic Dynamics

RNA sequence variants in live poliovirus vaccine and their relation to neurovirulence

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Chumakov, K M, Norwood, L P, Parker, M L, Dragunsky, E M, Ran, Y X, Levenbook, I S·1992·Journal of Virology

Abstract

Mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC) was used to study sequence heterogeneity and stability in attenuated poliovirus type 3 at positions in which the vaccine virus differs from its wild-type progenitor. Of seven genomic positions tested, only two (positions 472 and 2493) show nucleotide heterogeneity. Propagation of the vaccine virus in cell cultures leads to rapid selection of virus with reversions at these two positions of the genome. The relative abundance of reversions at position 472 correlates with the results of monkey neurovirulence tests, while the mutation at position 2493 is not directly associated with neurovirulence of the virus in monkeys. Instead, the abundance of mutations at the latter position correlates with the source of the seed virus and its passage level. These results further indicate that MAPREC at position 472 can be used to assess the quality of poliovirus type 3 vaccine.

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Registry

Crossref · 10.1128/jvi.66.2.966-970.1992

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5 of 5 references verified0 citation errors · 0 not checkable

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  • The key phenotypes of low neuropathogenicity for monkeys and temperature sensitivity are least stable for the Sabin 3 strain.

    “These phenotypes are least stable for the Sabin 3 strain (28), which has the highest variability across production lots and is also associated with the highest incidence of VAPP among OPV recipients (see below).”

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Authors
Chumakov, K. M., Norwood, L. P., Parker, M. L., Dragunsky, E. M., Ran, Y. X., & Levenbook, I. S.
Affiliations
Center for Biologics Evaluation and Research (US)
Venue
Journal of Virology
Published
1992-02
Volume
66
Issue
2
Pages
966-970
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
OpenAlex
W1517149542
Cited by
72
References
5
Citations by year
2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2018: 1 · 2017: 1 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 4
FWCI
3.30
Type
journal article
Language
en
Open access
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
oral polio vaccine, neurovirulence, reversion mutations
MeSH terms
Animals, Base Sequence, Macaca, Molecular Sequence Data, Mutation, Poliovirus Vaccine, Oral, Genetic Variation, Virulence, Restriction Mapping, Polymerase Chain Reaction, Genome, Viral, Poliovirus

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