The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G
Open accessAbstract
Currently, amphotropic retroviral vectors are widely used for gene transfer into CD34+ hematopoietic progenitor cells. The relatively low levels of transduction efficiency associated with these vectors in human cells is due to low viral titers and limitations in concentrating the virus because of the inherent fragility of retroviral envelopes. Here we show that a human immunodeficiency virus type 1 (HIV-1)-based retroviral vector containing the firefly luciferase reporter gene can be pseudotyped with a broad-host-range vesicular stomatitis virus envelope glycoprotein G (VSV-G). Higher-efficiency gene transfer into CD34+ cells was achieved with a VSV-G-pseudotyped HIV-1 vector than with a vector packaged in an amphotropic envelope. Concentration of virus without loss of viral infectivity permitted a higher multiplicity of infection, with a consequent higher efficiency of gene transfer, reaching 2.8 copies per cell. These vectors also showed remarkable stability during storage at 4 degrees C for a week. In addition, there was no significant loss of titer after freezing and thawing of the stock virus. The ability of VSV-G-pseudotyped retroviral vectors to achieve a severalfold increase in levels of transduction into CD34+ cells will allow high-efficiency gene transfer into hematopoietic progenitor cells for gene therapy purposes. Furthermore, since it has now become possible to infect CD34+ cells with pseudotyped HIV-1 with a high level of efficiency in vitro, many important questions regarding the effect of HIV-1 on lineage-specific differentiation of hematopoietic progenitors can now be addressed.
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Cites
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Cited by
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
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Generation of induced pluripotent stem cells from human blood
Loh, Y.-H., Agarwal, S., Park, I.-H., Urbach, A., Huo, H., Heffner, G. C., Kim, K., Miller, J. D., Ng, K., & Daley, G. Q. (2009) · Blood · doi:10.1182/blood-2009-02-204800 · Cited by 626
Generation of Induced Pluripotent Stem Cells from Human Cord Blood
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Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles
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Metadata
- Authors
- Akkina, R. K., Walton, R. M., Chen, M. L., Li, Q. X., Planelles, V, & Chen, I. S.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1996-04
- Volume
- 70
- Issue
- 4
- Pages
- 2581-2585
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1591189766
- Cited by
- 321
- References
- 24
- FWCI
- 9.23
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G. https://app.cochat.ai/papers/10.1128/jvi.70.4.2581-2585.1996
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- Akkina, R. K., Walton, R. M., Chen, M. L., Li, Q. X., Planelles, V, & Chen, I. S. (1996). High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G. Journal of Virology. https://doi.org/10.1128/jvi.70.4.2581-2585.1996
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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