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Cited by 561

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Proteolytic activation of tick-borne encephalitis virus by furin

Open access
Stadler, K, Allison, S L, Schalich, J, Heinz, F X·1997·Journal of Virology
DOI 10.1128/jvi.71.11.8475-8481.1997
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Abstract

Flaviviruses are assembled intracellularly in an immature form containing heterodimers of two envelope proteins, E and prM. Shortly before the virion exits the cell, prM is cleaved by a cellular enzyme, and this processing step can be blocked by treatment with agents that raise the pH of exocytic compartments. We carried out in vivo and in vitro studies with tick-borne encephalitis (TBE) virus to investigate the possible role of furin in this process as well as the functional consequences of prM cleavage. We found that prM in immature virions can be correctly cleaved in vitro by recombinant bovine furin but that efficient cleavage occurs only after exposure of the virion to mildly acidic pH. The data suggest that exposure to an acidic environment induces an irreversible structural change that renders the cleavage site accessible to the enzyme. Cleavage by furin in vitro resulted in biological activation, as shown by a 100-fold increase in specific infectivity, the acquisition of membrane fusion and hemagglutination activity, and the ability of the envelope proteins to undergo low-pH-induced structural rearrangements characteristic of mature virions. In vivo, prM cleavage was blocked by a furin inhibitor, and infection of the furin-deficient cell line LoVo yielded only immature virions, suggesting that furin is essential for cleavage activation of flaviviruses.

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Crossref · 10.1128/jvi.71.11.8475-8481.1997

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Metadata

Authors
Stadler, K., Allison, S. L., Schalich, J., & Heinz, F. X.
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1997-11
Volume
71
Issue
11
Pages
8475-8481
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.71.11.8475-8481.1997
OpenAlex
W2098687402
Cited by
561
References
38
FWCI
6.58
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Proteolytic activation of tick-borne encephalitis virus by furin. https://app.cochat.ai/papers/10.1128/jvi.71.11.8475-8481.1997
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Stadler, K., Allison, S. L., Schalich, J., & Heinz, F. X. (1997). Proteolytic activation of tick-borne encephalitis virus by furin. Journal of Virology. https://doi.org/10.1128/jvi.71.11.8475-8481.1997

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.