The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Ubiquitin Is Covalently Attached to the p6 Gag Proteins of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus and to the p12 Gag Protein of Moloney Murine Leukemia Virus
Open accessAbstract
Host proteins are incorporated into retroviral virions during assembly and budding. We have examined three retroviruses, human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), and Moloney murine leukemia virus (Mo-MuLV), for the presence of ubiquitin inside each of these virions. After a protease treatment to remove exterior viral as well as contaminating cellular proteins, the proteins remaining inside the virion were analyzed. The results presented here show that all three virions incorporate ubiquitin molecules at approximately 10% of the level of Gag found in virions. In addition to free ubiquitin, covalent ubiquitin-Gag complexes were detected, isolated, and characterized from all three viruses. Our immunoblot and protein sequencing results on treated virions showed that approximately 2% of either HIV-1 or SIV p6 Gag was covalently attached to a single ubiquitin molecule inside the respective virions and that approximately 2 to 5% of the p12 Gag in Mo-MuLV virions was monoubiquitinated. These results show that ubiquitination of Gag is conserved among these retroviruses and occurs in the p6 Gag portion of the Gag polyprotein, a region that is likely to be involved in assembly and budding.
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Cites
A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein
Chau, V., Tobias, J. W., Bachmair, A., Marriott, D., Ecker, D. J., Gonda, D. K., & Varshavsky, A. (1989) · Science · doi:10.1126/science.2538923 · Cited by 1,535
Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release
Göttlinger, H. G., Dorfman, T., Sodroski, J. G., & Haseltine, W. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.8.3195 · Cited by 647
Parent, L. J., Bennett, R. P., Craven, R. C., Nelle, T. D., Krishna, N. K., Bowzard, J. B., Wilson, C. B., Puffer, B. A., Montelaro, R. C., & Wills, J. W. (1995) · Journal of Virology · doi:10.1128/jvi.69.9.5455-5460.1995 · Cited by 258 · cited 1×
“This region in HIV-1 appears to be involved in assembly and budding (19, 26, 39).”
Background
Cited by
Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55 Gag
VerPlank, L., Bouamr, F., LaGrassa, T. J., Agresta, B., Kikonyogo, A., Leis, J., & Carter, C. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.131059198 · Cited by 584
Freed, E. O. (2002) · Journal of Virology · doi:10.1128/jvi.76.10.4679-4687.2002 · Cited by 425
Cellular Proteins in Influenza Virus Particles
Shaw, M. L., Stone, K. L., Colangelo, C. M., Gulcicek, E. E., & Palese, P. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000085 · Cited by 324
A role for ubiquitin ligase recruitment in retrovirus release
Strack, B., Calistri, A., Accola, M. A., Palù, G., & Göttlinger, H. G. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.24.13063 · Cited by 309
Ubiquitin is part of the retrovirus budding machinery
Patnaik, A., Chau, V., & Wills, J. W. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.24.13069 · Cited by 292
Metadata
- Authors
- Ott, D. E., Coren, L. V., Copeland, T. D., Kane, B. P., Johnson, D. G., Sowder, R. C., Yoshinaka, Y., Oroszlan, S., Arthur, L. O., & Henderson, L. E.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1998-04
- Volume
- 72
- Issue
- 4
- Pages
- 2962-2968
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2159011164
- Cited by
- 169
- References
- 3
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Ott, D. E., Coren, L. V., Copeland, T. D., Kane, B. P., Johnson, D. G., Sowder, R. C., Yoshinaka, Y., Oroszlan, S., Arthur, L. O., & Henderson, L. E. (1998). Ubiquitin Is Covalently Attached to the p6 Gag Proteins of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus and to the p12 Gag Protein of Moloney Murine Leukemia Virus. Journal of Virology. https://doi.org/10.1128/jvi.72.4.2962-2968.1998
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
