The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells
Open accessAbstract
Coronavirus contains three envelope proteins, M, E and S, and a nucleocapsid, which consists of genomic RNA and N protein, within the viral envelope. We studied the macromolecular interactions involved in coronavirus assembly in cells infected with a murine coronavirus, mouse hepatitis virus (MHV). Coimmunoprecipitation analyses demonstrated an interaction between N protein and M protein in infected cells. Pulse-labeling experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a pre-Golgi compartment, which is part of the MHV budding site. Coimmunoprecipitation analyses further revealed that M protein interacted with only genomic-length MHV mRNA, mRNA 1, while N protein interacted with all MHV mRNAs. These data indicated that M protein interacted with the nucleocapsid, consisting of N protein and mRNA 1, in infected cells. The M protein-nucleocapsid interaction occurred in the absence of S and E proteins. Intracellular M protein-N protein interaction was maintained after removal of viral RNAs by RNase treatment. However, the M protein-N protein interaction did not occur in cells coexpressing M protein and N protein alone. These data indicated that while the M protein-N protein interaction, which is independent of viral RNA, occurred in the M protein-nucleocapsid complex, some MHV function(s) was necessary for the initiation of M protein-nucleocapsid interaction. The M protein-nucleocapsid interaction, which occurred near or at the MHV budding site, most probably represented the process of specific packaging of the MHV genome into MHV particles.
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The Molecular Biology of Coronaviruses
Masters, P. S. (2006) · Advances in Virus Research · doi:10.1016/s0065-3527(06)66005-3 · Cited by 1,761
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Molecular Interactions in the Assembly of Coronaviruses
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“For coronaviruses, the N protein plays important roles during host cell entry and virus particle assembly and release (Hogue, 1995; Narayanan et al., 2000 Narayanan et al., , 2003 .”
Background
Metadata
- Authors
- Narayanan, K., Maeda, A., Maeda, J., & Makino, S.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2000-09
- Volume
- 74
- Issue
- 17
- Pages
- 8127-8134
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2110524431
- Cited by
- 255
- References
- 28
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- green
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