The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Mutations in the Yellow Fever Virus Nonstructural Protein NS2A Selectively Block Production of Infectious Particles
Open accessAbstract
Little is known about the function of flavivirus nonstructural protein NS2A. Two forms of NS2A are found in yellow fever virus-infected cells. Full-length NS2A (224 amino acids) is the product of cleavage at the NS1/2A and NS2A/2B sites. NS2Aα, a C-terminally truncated form of 190 amino acids, results from partial cleavage by the viral NS2B-3 serine protease at the sequence QK↓T within NS2A. Exchange of serine for lysine at this site (QKT→QST) blocks the production of both NS2Aα and infectious virus. The present study reveals that this defect is not at the level of RNA replication. Despite normal structural region processing, infectious particles containing genome RNA and capsid protein were not released from cells transfected with the mutant RNA. Nevertheless, production of subviral prM/M- and E-containing particles was unimpaired. The NS2A defect could be complemented in trans by providing NS1-2A or NS1-2Aα. However, trans complementation was not observed when the C-terminal lysine of NS1-2Aα was replaced with serine. In addition to true reversions, NS2Aα cleavage site mutations could be suppressed by two classes of second-site changes. The first class consisted of insertions at the NS2Aα cleavage site that restored its basic character and cleavability. A second class of suppressors occurred in the NS3 helicase domain, in which NS3 aspartate 343 was replaced with an uncharged residue (either valine, alanine, or glycine). These mutations in NS3 restored infectious-virus production in the absence of cleavage at the mutant NS2Aα site. Taken together, our results reveal an unexpected role for NS2A and NS3 in the assembly and/or release of infectious flavivirus particles.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Thompson, J. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.24.4876 · Cited by 38,993
Schägger, H., & von Jagow, G. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90587-2 · Cited by 11,444
FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATION
Chambers, T. J., Hahn, C. S., Galler, R., & Rice, C. M. (1990) · Annual Review of Microbiology · doi:10.1146/annurev.mi.44.100190.003245 · Cited by 2,014
Self-citationYao, N., Reichert, P., Taremi, S. S., Prosise, W. W., & Weber, P. C. (1999) · Structure · doi:10.1016/s0969-2126(00)80025-8 · Cited by 391
trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication
Lindenbach, B. D., & Rice, C. M. (1997) · Journal of Virology · doi:10.1128/jvi.71.12.9608-9617.1997 · Cited by 374
Self-citationMackenzie, J. M., Khromykh, A. A., Jones, M. K., & Westaway, E. G. (1998) · Virology · doi:10.1006/viro.1998.9156 · Cited by 326
Lindenbach, B. D., & Rice, C. M. (1999) · Journal of Virology · doi:10.1128/jvi.73.6.4611-4621.1999 · Cited by 308
Self-citationSerious adverse events associated with yellow fever 17DD vaccine in Brazil: a report of two cases
Vasconcelos, P. F., Luna, E. J., Galler, R., Silva, L. J., Coimbra, T. L., Barros, V. L., Monath, T. P., Rodigues, S. G., Laval, C., Costa, Z. G., et al. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05326-0 · Cited by 287
Cited by
Molecular biology of flaviviruses
Lindenbach, B. D., & Rice, C. M. (2003) · Advances in Virus Research · doi:10.1016/s0065-3527(03)59002-9 · Cited by 853
Self-citationDengue Virus Capsid Protein Usurps Lipid Droplets for Viral Particle Formation
Samsa, M. M., Mondotte, J. A., Iglesias, N. G., Assunção-Miranda, I., Barbosa-Lima, G., Da Poian, A. T., Bozza, P. T., & Gamarnik, A. V. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000632 · Cited by 586
Expression of the Zinc-Finger Antiviral Protein Inhibits Alphavirus Replication
Bick, M. J., Carroll, J.-W. N., Gao, G., Goff, S. P., Rice, C. M., & MacDonald, M. R. (2003) · Journal of Virology · doi:10.1128/jvi.77.21.11555-11562.2003 · Cited by 384
Self-citationBiochemistry and Molecular Biology of Flaviviruses
Barrows, N. J., Campos, R. K., Liao, K.-C., Prasanth, K. R., Soto-Acosta, R., Yeh, S.-C., Schott-Lerner, G., Pompon, J., Sessions, O. M., Bradrick, S. S., et al. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00719 · Cited by 341
Schuberth-Wagner, C., Ludwig, J., Bruder, A. K., Herzner, A.-M., Zillinger, T., Goldeck, M., Schmidt, T., Schmid-Burgk, J. L., Kerber, R., Wolter, S., et al. (2015) · Immunity · doi:10.1016/j.immuni.2015.06.015 · Cited by 311
Metadata
- Authors
- Kümmerer, B. M., & Rice, C. M.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2002-05-15
- Volume
- 76
- Issue
- 10
- Pages
- 4773-4784
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1985274927
- Cited by
- 301
- References
- 21
- FWCI
- 13.66
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Mutations in the Yellow Fever Virus Nonstructural Protein NS2A Selectively Block Production of Infectious Particles. https://app.cochat.ai/papers/10.1128/jvi.76.10.4773-4784.2002
- The paper
- Kümmerer, B. M., & Rice, C. M. (2002). Mutations in the Yellow Fever Virus Nonstructural Protein NS2A Selectively Block Production of Infectious Particles. Journal of Virology. https://doi.org/10.1128/jvi.76.10.4773-4784.2002
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
