Evidence for Packaging of rep-cap Sequences into Adeno-Associated Virus (AAV) Type 2 Capsids in the Absence of Inverted Terminal Repeats: a Model for Generation of rep -Positive AAV Particles
Open accessAbstract
We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.
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Rep expression cassettes driven by the AAV p5 promoter result in significant rep gene copackaging, possibly due to a cryptic RBE in the p5 promoter.
“Rep expression cassettes, driven by the AAV p5 promoter, have been reported to result in significant rep gene copackaging possibly due to the presence of a cryptic RBE in the p5 promoter [83].”
From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [83]
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“Several investigators have shown that AAV DNA and heterologous, chromosomal DNA that contains a Rep binding element and a terminal resolution site, but no TRs, can also be packaged into AAV virions (Huser et al., 2003; Nony et al., 2003).”
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- Authors
- Nony, P., Chadeuf, G., Tessier, J., Moullier, P., & Salvetti, A.
- Affiliations
- Inserm (FR)
- Corresponding author
- Salvetti, Anna
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2003-01
- Volume
- 77
- Issue
- 1
- Pages
- 776-781
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC140600
- OpenAlex
- W2112382843
- Cited by
- 51
- References
- 0
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2021: 3 · 2020: 6 · 2019: 3 · 2018: 1 · 2017: 5 · 2015: 4 · 2014: 3 · 2013: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- adeno-associated virus type 2, rep gene, inverted terminal repeats, cis-regulatory elements, viral assembly, AAV capsid
- MeSH terms
- Dependovirus, Capsid, DNA-Binding Proteins, Plasmids, Viral Proteins, Virion, Virus Assembly, Terminal Repeat Sequences
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