Neuraminidase Is Important for the Initiation of Influenza Virus Infection in Human Airway Epithelium
Open accessAbstract
Influenza virus neuraminidase (NA) plays an essential role in release and spread of progeny virions, following the intracellular viral replication cycle. To test whether NA could also facilitate virus entry into cell, we infected cultures of human airway epithelium with human and avian influenza viruses in the presence of the NA inhibitor oseltamivir carboxylate. Twenty- to 500-fold less cells became infected in drug-treated versus nontreated cultures ( P < 0.0001) 7 h after virus application, indicating that the drug suppressed the initiation of infection. These data demonstrate that viral NA plays a role early in infection, and they provide further rationale for the prophylactic use of NA inhibitors.
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Cites
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“For example, the sialic acid binding (“hemagglutinating”, H) activity of the influenza viruses is balanced by the activity of its sialic acid releasing enzyme (neuraminidase, N), the latter working both to allow the virus to gain access to cell surfaces by cutting through interfering molecules (788, 789), and also to…”
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“As for the influenza virus, its sialic acid-binding (the hemagglutinating, H) activity is balanced by the sialic acid-releasing enzyme (the neuraminidase, N) resulting in the cleavage of interfering molecules and accessibility of viruses to the cell surface [101,102].”
Background
- Authors
- Matrosovich, M. N., Matrosovich, T. Y., Gray, T., Roberts, N. A., & Klenk, H.-D.
- Affiliations
- Philipps University of Marburg (DE)
- National Institutes of Health (US)
- Roche (United Kingdom) (GB)
- Corresponding author
- Matrosovich, Mikhail N.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2004-11-15
- Volume
- 78
- Issue
- 22
- Pages
- 12665-12667
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC525087
- OpenAlex
- W2108358599
- Cited by
- 606
- References
- 12
- Citations by year
- 2026: 11 · 2025: 22 · 2024: 27 · 2023: 31 · 2022: 23 · 2021: 29 · 2020: 21 · 2019: 37 · 2018: 28 · 2017: 29 · 2016: 41 · 2015: 45 · 2014: 44 · 2013: 42 · 2012: 34
- FWCI
- 11.26
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Keywords
- neuraminidase, influenza virus, oseltamivir carboxylate, viral entry, airway epithelium, avian influenza virus, human influenza virus, prophylaxis
- MeSH terms
- Acetamides, Bronchi, Humans, Nasal Mucosa, Neuraminidase, Orthomyxoviridae, Trachea, Oseltamivir
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