CoChat
Sign Up
Cited by 51melanin and skin pigmentationMicroRNA in disease regulationMelanoma and MAPK Pathways

MicroRNA 340 is involved in UVB-induced dendrite formation through the regulation of RhoA expression in melanocytes

Open access
Jian, Qiang, An, Qing, Zhu, Dongning, Hui, Kun, Liu, Ying, Chi, Sumin, Li, Chengxin·2014·Molecular and Cellular Biology
DOI 10.1128/mcb.00106-14PMC PMC4135612
Open this paper in CoChat — free account

Abstract

The influence of UV irradiation on pigmentation is well established, but the molecular and cellular mechanisms controlling dendrite formation remain incompletely understood. MicroRNAs (miRNAs) are a class of small RNAs that participate in various cellular processes by suppressing the expression of target mRNAs. In this study, we investigated the expression of miRNAs in response to UVB irradiation using a microarray screen and then identified potential mRNA targets for differentially expressed miRNAs among the genes governing dendrite formation. We subsequently determined the ability of miRNA 340 (miR-340) to suppress the expression of RhoA, which is a predicted miR-340 target gene that regulates dendrite formation. The overexpression of miR-340 promoted dendrite formation and melanosome transport, and the downregulation of miR-340 inhibited UVB-induced dendrite formation and melanosome transport. Moreover, a luciferase reporter assay demonstrated direct targeting of RhoA by miR-340 in the immortalized human melanocyte cell line Pig1. In conclusion, this study has established an miRNA associated with UVB irradiation. The significant downregulation of RhoA protein and mRNA expression after UVB irradiation and the modulation of miR-340 expression suggest a key role for miR-340 in regulating UVB-induced dendrite formation and melanosome transport.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.00106-14

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • miR-340 promotes dendrite extension through suppressing RhoA expression in melanocytes.

    “Another RhoA-targeting miRNA linked to dendrite formation is miR-340, which has been shown to promote dendrite extension through suppressing RhoA expression in melanocytes [113].”

    From RhoA Signaling in Neurodegenerative Diseases (2022) · cites this paper as [113]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 49

  1. [1]1. Dorsky RI, Moon RT, Raible DW. 2000. Environmental signals and cell fate specification in premigratory neural crest. Bioessays 22: 708-716. http://dx.doi.org/10.1002/1521-1878(200008)22:8 < 708::AID-BIES4 > 3. 0.CO;2-N. · DOI
  2. [2]2. Yamaguchi Y, Brenner M, Hearing VJ. 2007. The regulation of skin pigmentation. J. Biol. Chem. 282: 27557-27561. http://dx.doi.org/10.1074 /jbc.R700026200.
  3. [3]3. Eller MS, Maeda T, Magnoni C, Atwal D, Gilchrest BA. 1997. Enhancement of DNArepair in human skin cells by thymidine dinucleotides: evidence for a p53-mediated mammalian SOS response. Proc. Natl. Acad. Sci. U. S. A. 94: 12627-12632. http://dx.doi.org/10.1073/pnas.94.23.12627. · DOI
  4. [4]4. Sulaimon SS, Kitchell BE. 2003. The biology of melanocytes. Vet. Dermatol. 14: 57-65. http://dx.doi.org/10.1046/j.1365-3164.2003.00327.x. · DOI
  5. [5]5. Iyengar B. 1992. Neural differentiation as an expression of UV sensitivity of melanocytes. Acta Anat. (Basel) 143: 236-240. http://dx.doi.org/10 .1159/000147254.
  6. [6]6. Iyengar B. 1994. UV guided dendritic growth patterns and the networking of melanocytes. Experientia 50: 669-672. http://dx.doi.org/10 .1007/BF01952870.
  7. [7]7. Duval C, Régnier M, Schmidt R. 2001. Distinct melanogenic response of human melanocytes in mono-culture, in co-culture with keratinocytes and in reconstructed epidermis, to UV exposure. Pigment Cell Res. 14: 348-355. http://dx.doi.org/10.1034/j.1600-0749.2001.140506.x. · DOI · Paper page
  8. [8]8. Scott G. 2002. Rac and rho: the story behind melanocyte dendrite formation. Pigment Cell Res. 15: 322-330. http://dx.doi.org/10.1034/j.1600 -0749.2002.02056.x. · DOI
  9. [9]9. Jimbow M, Jimbow K. 1989. Pigmentary disorders in oriental skin. Clin. Dermatol. 7: 11-27. http://dx.doi.org/10.1016/0738-081X(89)90053-9. · DOI
  10. [10]10. Pichardo R, Vallejos Q, Feldman SR, Schulz MR, Verma A, Quandt SA, Arcury TA. 2009. The prevalence of melasma and its association with quality of life in adult male Latino migrant workers. Int. J. Dermatol. 48: 22-26. http://dx.doi.org/10.1111/j.1365-4632.2009.03778.x. · DOI
  11. [11]11. Kang WH, Yoon KH, Lee ES, Kim J, Lee KB, Yim H, Sohn S, Im S. 2002. Melasma: histopathological characteristics in 56 Korean patients. Br. J. Dermatol. 146: 228-237. http://dx.doi.org/10.1046/j.0007-0963.2001.04556.x. · DOI
  12. [12]12. Miska EA. 2005. How microRNAs control cell division, differentiation anddeath. Curr. Opin. Genet. Dev. 15: 563-568. http://dx.doi.org/10.1016 /j.gde.2005.08.005.
  13. [13]13. Gaur A, Jewell DA, Liang Y, Ridzon D, Moore JH, Chen C, Ambros VR, Israel MA. 2007. Characterization of microRNA expression levels and their biological correlates in human cancer cell lines. Cancer Res. 67: 2456-2468. http://dx.doi.org/10.1158/0008-5472.CAN-06-2698. · DOI · Paper page
  14. [14]14. Gottwein E, Cullen BR. 2008. Viral and cellular microRNAs as determinants of viral pathogenesis and immunity. Cell Host Microbe 3: 375-387. http://dx.doi.org/10.1016/j.chom.2008.05.002. · DOI
  15. [16]16. Li T, Li D, Sha J, Sun P, Huang Y. 2009. MicroRNA-21 directly targets MARCKS and promotes apoptosis resistance and invasion in prostate cancer cells. Biochem. Biophys. Res. Commun. 383: 280-285. http://dx .doi.org/10.1016/j.bbrc.2009.03.077. · DOI
  16. [17]17. Hicks JA, Trakooljul N, Liu HC. 2010. Discovery of chicken microRNAs associated with lipogenesis and cell proliferation. Physiol. Genomics 41: 185-193. http://dx.doi.org/10.1152/physiolgenomics.00156.2009. · DOI
  17. [18]18. Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, Baskerville S, Hammond SM, Bartel DP, Schier AF. 2005. MicroRNAs regulate brain morphogenesis in zebrafish. Science 308: 833-838. http: //dx.doi.org/10.1126/science.1109020. · DOI · Paper page
  18. [19]19. Kim D, Song J, Kim S, Park HM, Chun CH, Sonn J, Jin EJ. 2012. MicroRNA-34a modulates cytoskeletal dynamics through regulating RhoA/Rac1 cross-talk in chondroblasts. J. Biol. Chem. 287: 12501-12509. http://dx.doi.org/10.1074/jbc.M111.264382. · DOI
  19. [20]20. Schratt GM, Tuebing F, Nigh EA, Kane CG, Sabatini ME, Kiebler M, Greenberg ME. 2006. A brain-specific microRNA regulates dendritic spine development. Nature 439: 283-289. http://dx.doi.org/10.1038 /nature04367.
  20. [21]21. Siegel G, Obernosterer G, Fiore R, Oehmen M, Bicker S, Christensen M, Khudayberdiev S, Leuschner PF, Busch CJ, Kane C, Hübel K, Dekker F, Hedberg C, Rengarajan B, Drepper C, Waldmann H, Kauppinen S, Greenberg ME, Draguhn A, Rehmsmeier M, Martinez J, Schratt GM. 2009. A functional screen implicates microRNA-138dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis. Nat. Cell Biol. 11: 705-716. http://dx.doi.org/10 .1038/ncb1876.
  21. [22]22. Impey S, Davare M, Lesiak A, Fortin D, Ando H, Varlamova O, Obrietan K, Soderling TR, Goodman RH, Wayman GA. 2010. An activity-induced microRNA controls dendritic spine formation by regulating Rac1-PAK signaling. Mol. Cell. Neurosci. 43: 146-156. http://dx.doi .org/10.1016/j.mcn.2009.10.005. · DOI · Paper page
  22. [23]23. Guo L, Huang ZX, Chen XW, Deng QK, Yan W, Zhou MJ, Ou CS, Ding ZH. 2009. Differential expression profiles of microRNAs in NIH3T3 cells in response to UVB irradiation. Photochem. Photobiol. 85: 765-773. http: //dx.doi.org/10.1111/j.1751-1097.2008.00482.x. · DOI
  23. [24]24. Pothof J, Verkaik NS, Hoeijmakers JH, van Gent DC. 2009. MicroRNA responses and stress granule formation modulate the DNA damage response. Cell Cycle 8: 3462-3468. http://dx.doi.org/10.4161/cc.8.21.9835. · DOI
  24. [25]25. Le Poole van den Berg FM, van den Wijngaard RM, Galloway DA, van Amstel PJ, Buffing AA, Smits HL, Westerhof W, Das PK. 1997. Generation of a human melanocyte cell line by introduction of HPV16 E6 and E7 genes. In Vitro Cell. Dev. Biol. Anim. 33: 42-49. http://dx.doi.org/10.1007 /s11626-997-0021-6.
  25. [26]26. Suzuki HI, Yamagata K, Sugimoto K, Iwamoto T, Kato S, Miyazono K. 2009. Modulation of microRNA processing by p53. Nature 460: 529-533. http://dx.doi.org/10.1038/nature08199. · DOI
  26. [27]27. Burridge K, Wennerberg K. 2004. Rho and Rac take center stage. Cell 116: 167-179. http://dx.doi.org/10.1016/S0092-8674(04)00003-0. · DOI
  27. [28]28. Kim MY, Choi TY, Kim JH, Lee JH, Kim JG, Sohn KC, Yoon KS, Kim CD, Lee JH, Yoon TJ. 2010. MKK6 increases the melanocyte dendricity - through the regulation of Rho family GTPases. J. Dermatol. Sci. 60: 114119. http://dx.doi.org/10.1016/j.jdermsci.2010.08.006. · DOI
  28. [29]29. Ito Y, Kanamaru A, Tada A. 2006. Centaureidin promotes dendrite retraction of melanocytes by activating Rho. Biochim. Biophys. Acta 1760: 487-494. http://dx.doi.org/10.1016/j.bbagen.2006.01.003. · DOI
  29. [30]30. Scott G, Leopardi S. 2003. The cAMP signaling pathway has opposing effects on Rac and Rho in B16F10 cells: implications for dendrite formation in melanocytic cells. Pigment Cell Res. 16: 139-148. http://dx.doi.org /10.1034/j.1600-0749.2003.00022.x. · DOI
  30. [31]31. Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T. 2003. The small RNA profile during Drosophila melanogaster development. Dev. Cell 5: 337-350. http://dx .doi.org/10.1016/S1534-5807(03)00228-4. · DOI
  31. [32]32. Fineberg SK, Kosik KS, Davidson BL. 2009. MicroRNAs potentiate neural development. Neuron 64: 303-309. http://dx.doi.org/10.1016/j .neuron.2009.10.020. · DOI
  32. [33]33. Chen JF, Mandel EM, Thomson JM, Wu Q, Callis TE, Hammond SM, Conlon FL, Wang DZ. 2006. The role of microRNA-1 and microRNA133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38: 228-233. http://dx.doi.org/10.1038/ng1725. · DOI
  33. [34]34. Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV, Sun Y, Koo S, Perera RJ, Jain R, Dean NM, Freier SM, Bennett CF, Lollo B, Griffey R. 2004. MicroRNA-143 regulates adipocyte differentiation. J. Biol. Chem. 279: 52361-52365. http://dx.doi.org/10.1074/jbc .C400438200. · DOI
  34. [35]35. Nakahara K, Carthew RW. 2004. Expanding roles for miRNAs and siRNAs in cell regulation. Curr. Opin. Cell Biol. 16: 127-133. http://dx.doi .org/10.1016/j.ceb.2004.02.006. · DOI
  35. [36]36. Mott JL, Kobayashi S, Bronk SF, Gores GJ. 2007. mir-29 regulates Mcl-1 protein expression and apoptosis. Oncogene 26: 6133-6140. http://dx.doi .org/10.1038/sj.onc.1210436. · DOI
  36. [37]37. Welch C, Chen Y, Stallings RL. 2007. MicroRNA-34a functions as a potential tumor suppressor by inducing apoptosis in neuroblastoma cells. Oncogene 26: 5017-5022. http://dx.doi.org/10.1038/sj.onc.1210293. · DOI
  37. [38]38. Hwang HW, Mendell JT. 2006. MicroRNAs in cell proliferation, cell death, and tumorigenesis. Br. J. Cancer 94: 776-780. http://dx.doi.org/10 .1038/sj.bjc.6603023.
  38. [39]39. Skaftnesmo KO, Prestegarden L, Micklem DR, Lorens JB. 2007. MicroRNAs in tumorigenesis. Curr. Pharm. Biotechnol. 8: 320-325. http: //dx.doi.org/10.2174/138920107783018390. · DOI
  39. [40]40. Kasinath BS, Feliers D, Sataranatarajan K, Ghosh Choudhury G, Lee MJ, Mariappan MM. 2009. Regulation of mRNA translation in renal - physiology and disease. Am. J. Physiol. Renal Physiol. 297: F1153-F1165. http://dx.doi.org/10.1152/ajprenal.90748.2008. · DOI
  40. [41]41. Martino S, di Girolamo I, Orlacchio A, Datti A, Orlacchio A. 2009. MicroRNA implications across neurodevelopment and neuropathology. J. Biomed. Biotechnol. 2009: 654346. http://dx.doi.org/10.1155/2009/654346. · DOI
  41. [42]42. Goswami S, Tarapore RS, Teslaa JJ, Grinblat Y, Setaluri V, Spiegelman VS. 2010. MicroRNA-340-mediated degradation of microphthalmiaassociated transcription factor mRNA is inhibited by the coding region determinant-binding protein. J. Biol. Chem. 285: 20532-20540. http://dx .doi.org/10.1074/jbc.M110.109298. · DOI
  42. [43]43. Wu ZS, Wu Q, Wang CQ, Wang XN, Huang J, Zhao JJ, Mao SS, Zhang GH, Xu XC, Zhang N. 2011. miR-340 inhibition of breast cancer cell migration and invasion through targeting of oncoprotein c-Met. Cancer 117: 2842-2852. http://dx.doi.org/10.1002/cncr.25860. · DOI
  43. [45]45. Zhou X, Wei M, Wang W. 2013. MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1. Biochem. Biophys. Res. Commun. 437: 653-658. http://dx.doi.org/10 .1016/j.bbrc.2013.07.033.
  44. [46]46. Suzuki HI, Miyazono K. 2013. p53 actions on microRNA expression and maturation pathway. Methods Mol. Biol. 962: 165-181. http://dx.doi.org /10.1007/978-1-62703-236-0\_14. · DOI
  45. [47]47. Buscà R, Bertolotto C, Abbe P, Englaro W, Ishizaki T, Narumiya S, Boquet P, Ortonne JP, Ballotti R. 1998. Inhibition of Rho is required for cAMP-induced melanoma cell differentiation. Mol. Biol. Cell 9: 13671378. http://dx.doi.org/10.1091/mbc.9.6.1367. · DOI
  46. [48]48. Katoh H, Aoki J, Ichikawa A, Negishi M. 1998. p160 RhoA-binding kinase ROKalpha induces neurite retraction. J. Biol. Chem. 273: 24892492. http://dx.doi.org/10.1074/jbc.273.5.2489. · DOI
  47. [49]49. Scott GA, Cassidy L. 1998. Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model. J. Investig. Dermatol. 111: 243-250. http://dx.doi.org/10 .1046/j.1523-1747.1998.00276.x.
  48. [50]50. Sander EE, ten Klooster JP, van Delft S, van der Kammen RA, Collard JG. 1999. Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J. Cell Biol. 147: 1009-1022. http://dx.doi.org/10.1083/jcb.147.5.1009. · DOI
  49. [51]51. Fan YM, Pang CP, Harvey AR, Cui Q. 2008. Marked effect of RhoAspecific shRNA-producing plasmids on neurite growth in PC12 cells. Neurosci. Lett. 440: 170-175. http://dx.doi.org/10.1016/j.neulet.2008.05 .045. · DOI

Cited by

Sorted by citations

Authors
Jian, Q., An, Q., Zhu, D., Hui, K., Liu, Y., Chi, S., & Li, C.
Affiliations
  • Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China
  • Xijing Hospital (CN)
  • Shaanxi Provincial Hospital of Traditional Chinese Medicine (CN)
  • Air Force Medical University (CN)
  • Chinese PLA General Hospital (CN)
ORCID
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
2014-09-01
Volume
34
Issue
18
Pages
3407-3420
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.00106-14
PMC
PMC4135612
OpenAlex
W2126408367
Cited by
51
References
1
Citations by year
2024: 5 · 2023: 6 · 2022: 1 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 6 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 3
FWCI
2.46
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
Keywords
miR-340, microRNA, UVB irradiation, dendrite formation, RhoA, melanocytes, melanosome transport, skin pigmentation, microRNA target regulation
MeSH terms
Base Sequence, Binding Sites, Cell Line, Dendrites, Gene Expression Regulation, Humans, Melanocytes, Molecular Sequence Data, RNA, Messenger, Skin Pigmentation, Sequence Alignment, Oligonucleotide Array Sequence Analysis, rhoA GTP-Binding Protein, cdc42 GTP-Binding Protein, MicroRNAs, rho-Associated Kinases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review