MicroRNAs Regulate Brain Morphogenesis in Zebrafish
Abstract
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZ dicer ) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZ dicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZ dicer mutants, revealing essential roles for miRNAs during morphogenesis.
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Maternal-zygotic dicer mutant zebrafish that lack all mature miRNAs display abnormal brain morphogenesis and neural differentiation.
“Maternal-zygotic dicer mutant zebrafish that lack all mature miRNAs display abnormal brain morphogenesis and neural differentiation (Giraldez et al. 2005).”
From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Giraldez et al. 2005
Injection of miR-430 rescues the brain defects in dicer mutant zebrafish embryos, inferring a general role in zebrafish brain morphogenesis.
“Notably, injection of miR-430 rescues the brain defects in the mutant embryos, inferring a general role in zebrafish brain morphogenesis.”
From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Giraldez et al. 2005
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- Authors
- Giraldez, A. J., Cinalli, R. M., Glasner, M. E., Enright, A. J., Thomson, J. M., Baskerville, S., Hammond, S. M., Bartel, D. P., & Schier, A. F.
- Affiliations
- Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
- Venue
- Science
- Published
- 2005-05-06
- Volume
- 308
- Issue
- 5723
- Pages
- 833-838
- ISSN
- 0036-8075
- 1095-9203
- Cited by
- 1,124
- References
- 1
- Language
- en
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