The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Activation of p53-Dependent Growth Suppression in Human Cells by Mutations in PTEN or PIK3CA
Open accessAbstract
In an effort to identify genes whose expression is regulated by activated phosphatidylinositol 3-kinase (PI3K) signaling, we performed microarray analysis and subsequent quantitative reverse transcription-PCR on an isogenic set of PTEN gene-targeted human cancer cells. Numerous p53 effectors were upregulated following PTEN deletion, including p21, GDF15, PIG3, NOXA, and PLK2. Stable depletion of p53 led to reversion of the gene expression program. Western blots revealed that p53 was stabilized in HCT116 PTEN(-/-) cells via an Akt1-dependent and p14(ARF)-independent mechanism. Stable depletion of PTEN in untransformed human fibroblasts and epithelial cells also led to upregulation of p53 and senescence-like growth arrest. Simultaneous depletion of p53 rescued this phenotype, enabling PTEN-depleted cells to continue proliferating. Next, we tested whether oncogenic PIK3CA, like inactivated PTEN, could activate p53. Retroviral expression of oncogenic human PIK3CA in MCF10A cells led to activation of p53 and upregulation of p53-regulated genes. Stable depletion of p53 reversed these PIK3CA-induced expression changes and synergized with oncogenic PIK3CA in inducing anchorage-independent growth. Finally, targeted deletion of an endogenous allele of oncogenic, but not wild-type, PIK3CA in a human cancer cell line led to a reduction in p53 levels and a decrease in the expression of p53-regulated genes. These studies demonstrate that activation of PI3K signaling by mutations in PTEN or PIK3CA can lead to activation of p53-mediated growth suppression in human cells, indicating that p53 can function as a brake on phosphatidylinositol (3,4,5)-triphosphate-induced mitogenesis during human cancer pathogenesis.
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Cites
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Self-citationMutant PIK3CA promotes cell growth and invasion of human cancer cells.
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Cited by
PI3K pathway alterations in cancer: variations on a theme
Yuan, T. L., & Cantley, L. C. (2008) · Oncogene · doi:10.1038/onc.2008.245 · Cited by 1,845
PTEN and the PI3-Kinase Pathway in Cancer
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p53 regulates epithelial–mesenchymal transition and stem cell properties through modulating miRNAs
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The PI3K/AKT/mTOR interactive pathway
Ersahin, T., Tuncbag, N., & Cetin-Atalay, R. (2015) · Molecular BioSystems · doi:10.1039/c5mb00101c · Cited by 683
Parkin‐independent mitophagy requires Drp1 and maintains the integrity of mammalian heart and brain
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Metadata
- Authors
- Kim, J.-S., Lee, C., Bonifant, C. L., Ressom, H., & Waldman, T.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2007-01-01
- Volume
- 27
- Issue
- 2
- Pages
- 662-677
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2131969649
- Cited by
- 161
- References
- 12
- FWCI
- 5.74
- Type
- journal article
- Language
- en
- Open access
- green
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- Kim, J.-S., Lee, C., Bonifant, C. L., Ressom, H., & Waldman, T. (2007). Activation of p53-Dependent Growth Suppression in Human Cells by Mutations in PTEN or PIK3CA. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.00537-06
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