The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Human AlkB Homolog ABH8 Is a tRNA Methyltransferase Required for Wobble Uridine Modification and DNA Damage Survival
Open accessAbstract
tRNA nucleosides are extensively modified to ensure their proper function in translation. However, many of the enzymes responsible for tRNA modifications in mammals await identification. Here, we show that human AlkB homolog 8 (ABH8) catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine (mcm(5)U) at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival. We find that ABH8 interacts specifically with tRNAs containing mcm(5)U and that purified ABH8 complexes methylate RNA in vitro. Significantly, ABH8 depletion in human cells reduces endogenous levels of mcm(5)U in RNA and increases cellular sensitivity to DNA-damaging agents. Moreover, DNA-damaging agents induce ABH8 expression in an ATM-dependent manner. These results expand the role of mammalian AlkB proteins beyond that of direct DNA repair and support a regulatory mechanism in the DNA damage response pathway involving modulation of tRNA modification.
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Cites
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Aas, P. A., Otterlei, M., Falnes, P. Ø., Vågbø, C. B., Skorpen, F., Akbari, M., Sundheim, O., Bjørås, M., Slupphaug, G., Seeberg, E., et al. (2003) · Nature · doi:10.1038/nature01363 · Cited by 638
Genes required for ionizing radiation resistance in yeast
Bennett, C. B., Lewis, L. K., Karthikeyan, G., Lobachev, K. S., Jin, Y. H., Sterling, J. F., Snipe, J. R., & Resnick, M. A. (2001) · Nature Genetics · doi:10.1038/ng778 · Cited by 328
Non-heme dioxygenases: cellular sensors and regulators jelly rolled into one?
Ozer, A., & Bruick, R. K. (2007) · Nature Chemical Biology · doi:10.1038/nchembio863 · Cited by 214
AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation
Ougland, R., Zhang, C.-M., Liiv, A., Johansen, R. F., Seeberg, E., Hou, Y.-M., Remme, J., & Falnes, P. Ø. (2004) · Molecular Cell · doi:10.1016/j.molcel.2004.09.002 · Cited by 205
Cited by
Fedeles, B. I., Singh, V., Delaney, J. C., Li, D., & Essigmann, J. M. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.656462 · Cited by 416
New frontiers in translational control of the cancer genome
Truitt, M. L., & Ruggero, D. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2016.27 · Cited by 378
DNA Repair by Reversal of DNA Damage
Yi, C., & He, C. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012575 · Cited by 161
Zhang, K., Eldin, P., Ciesla, J. H., Briant, L., Lentini, J. M., Ramos, J., Cobb, J., Munger, J., & Fu, D. (2024) · eLife · doi:10.7554/elife.90316.3 · Cited by 9
Self-citationZhang, K., Eldin, P., Ciesla, J. H., Briant, L., Lentini, J. M., Ramos, J., Cobb, J., Munger, J., & Fu, D. (2023) · doi:10.1101/2023.02.10.527147 · Cited by 3
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Metadata
- Authors
- Fu, D., Brophy, J. A. N., Chan, C. T. Y., Atmore, K. A., Begley, U., Paules, R. S., Dedon, P. C., Begley, T. J., & Samson, L. D.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2010-05-01
- Volume
- 30
- Issue
- 10
- Pages
- 2449-2459
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2150038630
- Cited by
- 210
- References
- 4
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Human AlkB Homolog ABH8 Is a tRNA Methyltransferase Required for Wobble Uridine Modification and DNA Damage Survival. https://app.cochat.ai/papers/10.1128/mcb.01604-09
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- Fu, D., Brophy, J. A. N., Chan, C. T. Y., Atmore, K. A., Begley, U., Paules, R. S., Dedon, P. C., Begley, T. J., & Samson, L. D. (2010). Human AlkB Homolog ABH8 Is a tRNA Methyltransferase Required for Wobble Uridine Modification and DNA Damage Survival. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.01604-09
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