The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes.
Open accessAbstract
Matrix attachment regions (MARs) are DNA elements that dissect the genome into topologically separated domains by binding to a chromosomal skeleton. This study explored the putative influence of the MAR located 5' of the chicken lysozyme gene on expression of heterologous genes in heterologous cell systems. Expression of a construct with the chloramphenicol acetyltransferase (CAT) indicator gene controlled by the herpes simplex virus thymidine kinase promoter (TC) and a construct in which the same transcriptional unit is flanked by chicken lysozyme 5' MARs (MTCM) was assayed after stable transfection into rat fibroblasts. Median CAT activity per copy number in MTCM transfectants was elevated approximately 10-fold relative to that in TC transfectants. Total variation in normalized CAT activity decreased from more than 100-fold among TC transfectants to nearly 6-fold among MTCM transfectants. The steady-state level of transcripts and the relative rate of transcription were increased in MTCM transfectants, as shown by S1 nuclease and run-on transcription assays, respectively. The chicken lysozyme 5' MAR thus can confer elevated, less position-dependent expression on a heterologous promoter in cells of a different species by increasing the density of transcribing RNA polymerase molecules. MAR-mediated transcriptional enhancement suggests that MARs are important for gene expression and not just for DNA packaging.
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Cited by
Biological Significance of Unwinding Capability of Nuclear Matrix-Associating DNAs
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Using matrix attachment regions to improve recombinant protein production
Harraghy, N., Buceta, M., Regamey, A., Girod, P.-A., & Mermod, N. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-352-3_7 · Cited by 30
Metadata
- Authors
- Phi-Van, L., von Kries, J. P., Ostertag, W., & Strätling, W. H.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1990-05
- Volume
- 10
- Issue
- 5
- Pages
- 2302-2307
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1822511846
- Cited by
- 275
- References
- 29
- FWCI
- 6.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Phi-Van, L., von Kries, J. P., Ostertag, W., & Strätling, W. H. (1990). The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.10.5.2302
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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